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Variation in Plant Morphology and Sinigrin Content in Ethiopian Mustard (Brassica carinata L.)
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2019-07-30 , DOI: 10.1016/j.hpj.2019.07.005
Tesfay Teklehaymanot , Haijing Wang , Jianli Liang , Jian Wu , Runmao Lin , Zhen Zhou , Xu Cai , Xiaowu Wang

Ethiopian mustard (Brassica carinata) is mainly grown as a leafy vegetable and oilseed crop. Sinigrin is predominant glucosinolate in the leaves of Ethiopian mustard. It is hydrolyzed by enzyme myrosinase to produce bioactive product specifically allyl-isothiocyanates. Due to sinigrin content, Ethiopian mustard has a mild flavor and it is a healthy addition to human diet. However, sinigrin concentration depends on genotype. Understanding variations of plant morphology and sinigrin content may be the first step for crop improvement. According to the analyses of various plant characteristics among ninety nine Ethiopian mustard accessions, we found significant differences in plant height, chlorophyll content, and petiole length. In addition, sinigrin content was determined using ultra-performance liquid chromatography tandem quadrupole mass spectrometers/tunable ultraviolet detector (UPLC-TQS/TUV). We found a significant difference in sinigrin content among leaves of 94 Ethiopian mustard accessions. Except for petiole length, the sinigrin content was negatively correlated with leaf area, leaf width, number of primary branches, and plant height. This information will help Ethiopian mustard breeders in screening breeding lines for new accessions.



中文翻译:

埃塞俄比亚芥菜(芥菜)中植物形态和芥子苷含量的变化

埃塞俄比亚芥末(芸苔)主要以叶菜类和油料作物的形式种植。Sinigrin是埃塞俄比亚芥末叶中主要的芥子油苷。它被黑芥子酶水解,产生生物活性产物,特别是烯丙基异硫氰酸酯。由于芥子苷的含量,埃塞俄比亚芥末味温和,是人类饮食中的健康补充。但是,芥子苷的浓度取决于基因型。了解植物形态和芥子苷含量的变化可能是作物改良的第一步。根据对九十九个埃塞俄比亚芥菜种的各种植物特征的分析,我们发现植物高度,叶绿素含量和叶柄长度存在显着差异。此外,使用超高效液相色谱串联四极杆质谱仪/可调紫外检测器(UPLC-TQS / TUV)测定芥子苷的含量。我们发现94种埃塞俄比亚芥菜种的叶片中芥子苷含量存在显着差异。除叶柄长度外,芥子苷的含量与叶面积,叶宽,初生枝数和株高呈负相关。这些信息将帮助埃塞俄比亚芥菜育种家筛选育种系以寻找新的种质。

更新日期:2019-07-30
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