Abstract Eisenia nordenskioldi is an earthworm widespread in Northern Asia and adjacent regions. It is known for its high morphological, karyotypic, and genetic variation, and contains two subspecies, the pigmented E. n. nordenskioldi and the unpigmented E. n. pallida. We obtained almost complete sequences of mitochondrial genomes (without the control region and parts of the flanking tRNAs) for six genetic lineages of E. n. nordenskioldi, two for E. n. pallida, as well as for three congeneric outgroups. These genomes had gene content and arrangement typical for Annelida. Nucleotide and amino acid diversity among E. nordenskioldi lineages was almost as high as between them and the outgroup species. E. nordenskioldi was split into two clades, one containing E. n. nordenskioldi lineages 6 and 9, and the other comprised the rest of the lineages. We could not resolve relationships of these two clades with E. tracta; most datasets used recovered this species as the sister group to E. n. nordenskioldi lineages 6 and 9, but not with high statistical support. According to mtDNA data, neither E. n. nordenskioldi nor E. n. pallida are monophyletic, and the same could be true for E. nordenskioldi as a whole, which suggest a revision of the systematics of E. nordenskioldi complex.

中文翻译：

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基于线粒体基因组的 Eisenia nordenskioldi 复合体的系统发育

摘要 Eisenia nordenskioldi 是一种广泛分布于北亚及邻近地区的蚯蚓。它以其高度的形态、核型和遗传变异而闻名，并包含两个亚种，即有色 E. n. nordenskioldi 和未染色的 E. n. 帕利达。我们获得了 E. n. 的六个遗传谱系的几乎完整的线粒体基因组序列（没有控制区域和侧翼 tRNA 的部分）。nordenskioldi，两个 E. n. pallida 以及三个同类外群。这些基因组具有Annelida 典型的基因内容和排列。E. nordenskioldi 谱系之间的核苷酸和氨基酸多样性几乎与它们与外群物种之间的一样高。E. nordenskioldi 被分成两个分支，一个包含 E. n. nordenskioldi 谱系 6 和 9，另一个包括其余谱系。我们无法解决这两个进化枝与 E.tracta 的关系；大多数使用的数据集将该物种恢复为 E. n. 的姊妹组。nordenskioldi 谱系 6 和 9，但没有很高的统计支持。根据 mtDNA 数据，E. n. nordenskioldi 或 E. n. pallida 是单系的，对于 E. nordenskioldi 整体而言也是如此，这表明对 E. nordenskioldi 复合体的系统学进行了修订。