当前位置: X-MOL 学术Eur. J. Soil Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biochar application changed arylsulfatase activity, kinetic and thermodynamic aspects
European Journal of Soil Biology ( IF 3.7 ) Pub Date : 2019-11-01 , DOI: 10.1016/j.ejsobi.2019.103134
Allahyar Khadem , Hossein Besharati , Mohammad Ali Khalaj

Abstract The aim of this research was to evaluate the response of soil arylsulfatase (ARS) kinetic and thermodynamic parameters to biochar application. The kinetic parameters including Vmax, Km and catalytic efficiency (Vmax Km−1) as well as thermodynamic ones (Ea, ΔHa and Q10) were determined in two different textured clayey and sandy loam soils amended with 0.5 and 1.0% (w/w) of unpyrolyzed maize residue (UMR) or its biochars prepared at 200 and 600 °C (B200 and B600, respectively). The results showed that the application of amendments to sandy loam soil increased enzymatic activity (16–106%), Km (37–113%) and the enzyme concentration (Vmax) (13–39%), whereas the Vmax Km−1 (38–86%), Ea (6–21%) and Q10 (8–11%) were decreased in the biochar-amended soils compared to the unamended control (CK). In clayey soil, biochar addition increased ARS activity (16–150%) and decreased the Vmax (10–41%), Vmax Km−1 (63–355%), Ea (20–63%) and Q10 (10–32%) and had no significant effect on Km. Generally, the effects of the amendments application were greater at 1% than 0.5% application rate and in clayey than sandy loam soil. Soil texture is a determinant factor in soil arylsulfatase response (activity, kinetics and thermodynamics) to biochar application.

中文翻译:

生物炭的应用改变了芳基硫酸酯酶的活性、动力学和热力学方面

摘要 本研究的目的是评估土壤芳基硫酸酯酶 (ARS) 动力学和热力学参数对生物炭应用的响应。动力学参数包括 Vmax、Km 和催化效率 (Vmax Km-1) 以及热力学参数 (Ea、ΔHa 和 Q10) 在两种不同质地的粘土和砂壤土中测定,分别添加 0.5% 和 1.0% (w/w)在 200 和 600 °C(分别为 B200 和 B600)下制备的未热解玉米残渣 (UMR) 或其生物炭。结果表明,对沙壤土施用改良剂可提高酶活性(16-106%)、Km(37-113%)和酶浓度(Vmax)(13-39%),而 Vmax Km-1( 38-86%)、Ea (6-21%) 和 Q10 (8-11%) 在生物炭改性土壤中与未改性对照 (CK) 相比降低。在黏性土壤中,biochar 添加增加了 ARS 活性 (16-150%) 并降低了 Vmax (10-41%)、Vmax Km-1 (63-355%)、Ea (20-63%) 和 Q10 (10-32%) 并降低了Km 无显着影响。一般来说,在 1% 的施用率和 0.5% 的施用率和黏性土壤中,改良剂施用的效果大于砂壤土。土壤质地是土壤芳基硫酸酯酶响应(活性、动力学和热力学)对生物炭应用的决定因素。
更新日期:2019-11-01
down
wechat
bug