Introduction

Effect of water stress on the physiology and biochemistry of two different Coleus species, Coleus forskholii and Coleus amboinicus, was studied.

Materials and methods

Drought stress was imposed by withholding the water supply until leaf water potentials reached −0.4, −0.8, and −1.2 MPa. Physiological parameters such as relative water content and water uptake capacity were studied along with lipid peroxidation, superoxide, H₂O₂, and ^•OH accumulation-, l-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging assays. Antioxidant defense system in Coleus under drought stress was studied by quantifying the Trolox equivalent antioxidant capacity, ascorbic acid, reduced glutathione-, and α-tocopherol content as well as activities of superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and glutathione reductase. Accumulation of osmolytes proline, glycine betaine, and phytohormone abscisic acid was also used as key parameters for assessing their performance.

Results

There was a marked variation in the antioxidative defense system and osmolyte accumulation in these two species under drought stress. Relative water content was reduced and water uptake capacity was increased.

Discussion

A comparative study in the perspectives of osmolyte accumulation, antioxidant, and physiological responses inferred C. amboinicus as a drought stress-tolerant species when compared to C. forskholii.

中文翻译：

---

水分胁迫对两种不同锦紫苏属植物生理生化反应的影响

介绍

研究了水分胁迫对两种不同Coleus物种Coleus forskholii和Coleus amboinicus生理和生物化学的影响。

材料和方法

干旱胁迫是通过暂停供水直到叶水势达到 -0.4、-0.8 和 -1.2 MPa 来施加的。生理参数如相对水含量和吸水能力与脂质过氧化、超氧化物、H ₂ O ₂和^• OH 积累-、l-二苯基-2-苦基肼自由基 (DPPH) 清除试验一起进行了研究。锦紫苏抗氧化防御系统通过量化 Trolox 等效抗氧化能力、抗坏血酸、还原型谷胱甘肽和 α-生育酚含量以及超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、过氧化物酶和谷胱甘肽还原酶的活性，研究了干旱胁迫下的干旱胁迫。渗透物脯氨酸、甘氨酸甜菜碱和植物激素脱落酸的积累也被用作评估其性能的关键参数。

结果

在干旱胁迫下，这两个物种的抗氧化防御系统和渗透剂积累存在显着差异。相对含水量减少，吸水能力增加。

讨论

与 C. forskholii相比，从渗透物积累、抗氧化和生理反应的角度进行的比较研究推断，C. amboinicus是耐干旱胁迫的物种。