Introduction

Several Aristolochia species were used as medicinal herb across Europe and in recent years, their antimicrobial activity has also been investigated.

Materials and methods

In this study, A. clematitis was selected to evaluate the aristolochic acids I and II (AA I and AA II) concentrations and the antimicrobial activity of methanol, hexane, butanol, and ethyl acetate extracts of the root, stem, leaf, root, and fruit. AA I and AA II contents were measured by a validated high-performance liquid chromatography–ultraviolet method.

Results

Each fraction of the plant contained AA I and AA II and the root was found to have the highest contents of AA I (1.09%) and AA II (0.7454%). The minimum inhibitory concentrations of all extracts were determined by standard microdilution method. The fruit’s extracts showed the most efficient antimicrobial effect against both methicillin sensitive and resistant Staphylococcus aureus strains.

Conclusion

Correlation between the AA I and AA II concentrations and the antimicrobial effect was not found.

中文翻译：

---

马兜铃酸的分析及抗菌活性评价。

介绍

几种马兜铃属植物在欧洲被用作药草，近年来，它们的抗菌活性也得到了研究。

材料和方法

在本研究中，选择A. clematitis来评估马兜铃酸 I 和 II（AA I 和 AA II）浓度以及甲醇、己烷、丁醇和乙酸乙酯提取物的根、茎、叶、根、和水果。A I 和 AA II 的含量通过一种经过验证的高效液相色谱-紫外法测定。

结果

植物的每个部分都含有 A I 和 AA II，发现根部的 A I (1.09%) 和 AA II (0.7454%) 含量最高。所有提取物的最低抑菌浓度通过标准微量稀释法测定。果实的提取物对甲氧西林敏感和耐药的金黄色葡萄球菌菌株均显示出最有效的抗菌作用。

结论

未发现 A I 和 AA II 浓度与抗菌作用之间的相关性。