Background and aims

The aim of this study was to verify that the comet assay can be used to investigate the DNA damaging effects of T-2 and HT-2 toxins in the liver of broiler chickens. The comet assay is a favorable genotoxic analysis because it is cheap, simple, and can be used in many organisms and different tissues.

Materials and methods

Male broiler chickens were fed with T-2/HT-2 toxins-contaminated diet for 14 days. The comet assay was successfully adapted to chicken liver cells, and the DNA damage was determined by a decrease in the comet parameter (DNA % in the tail) in the experimental groups.

Results

The method of evaluation was found to be critical because DNA damage could not be detected exactly using the CometScore software, due to inaccurate separation of head and comet. However, this problem can be solved by visual evaluation.

Conclusion

In the case of the visual evaluation, each toxin-treated group differed significantly from the control group, indicating that the assay can be useful for the assessment of primary DNA damage caused by T-2/HT-2 toxins.

中文翻译：

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T-2和HT-2毒素对鸡肝细胞遗传毒性效应的彗星实验研究

背景和目标

本研究的目的是验证彗星试验可用于研究 T-2 和 HT-2 毒素对肉鸡肝脏的 DNA 损伤作用。彗星试验是一种有利的基因毒性分析，因为它便宜、简单，并且可用于许多生物体和不同组织。

材料和方法

用受 T-2/HT-2 毒素污染的饲料喂养雄性肉鸡 14 天。彗星试验成功地适用于鸡肝细胞，并且通过实验组中彗星参数（尾部的 DNA %）的降低来确定 DNA 损伤。

结果

评估方法被认为是至关重要的，因为由于头部和彗星的分离不准确，使用 CometScore 软件无法准确检测 DNA 损伤。然而，这个问题可以通过视觉评估来解决。

结论

在视觉评估的情况下，每个毒素处理组与对照组有显着差异，表明该测定可用于评估由 T-2/HT-2 毒素引起的原发性 DNA 损伤。