Traditional two-dimensional (2D) cell cultures lack the extracellular matrix (ECM)-like structure or dynamic fluidic microenvironment for cells to maintain in vivo functionality. Three-dimensional (3D) tissue scaffolds, on the other hand, could provide the ECM-like microenvironment for cells to reformulate into tissue or organoids that are highly useful for in vitro drug screening. In this study, a high-throughput two-chamber 3D microscale tissue model platform is developed. Porous scaffolds are selectively foamed on a commercially available compact disk using laser. Perfusion of cell culture medium is achieved with centrifugal force-driven diffusion by disk rotation. Experimental studies were conducted on the fabrication process under various gas saturation and laser power conditions. Cell cultures were performed with two types of human cell lines: M059K and C3A-sub28. It is shown that the structure of microscale porous scaffolds can be controlled with laser foaming parameters and that coating with polydopamine these scaffolds are inducive for cell attachment and aggregation, forming a 3D network. With many such two-chamber models fabricated on a single CD and perfusion driven by the centrifugal force from rotation, the proposed platform provides a simple solution to the high-cost and lengthy drug development process with a high-throughput and physiologically more relevant tissue model system.

中文翻译：

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用于药物筛选的高通量三维细胞培养平台

传统的二维（2D）细胞培养物缺乏细胞外基质（ECM）样结构或动态流体微环境，细胞无法维持体内功能。另一方面，三维（3D）组织支架可以为细胞提供类似于ECM的微环境，以使细胞重新形成组织或类器官，这对于体外药物筛选非常有用。在这项研究中，开发了一种高通量的两室3D微型组织模型平台。使用激光将多孔支架选择性地发泡在可商购的光盘上。通过盘旋转通过离心力驱动的扩散来实现细胞培养基的灌注。在各种气体饱和度和激光功率条件下对制造工艺进行了实验研究。用两种类型的人类细胞系进行细胞培养：M059K和C3A-sub28。结果表明，可以用激光发泡参数控制微米级多孔支架的结构，用聚多巴胺包被这些支架可诱导细胞附着和聚集，形成3D网络。通过在单个CD上制造许多这样的两腔模型，并通过旋转的离心力驱动灌注，所提出的平台提供了一种高通量和生理相关性更高的组织模型，为高成本，冗长的药物开发过程提供了简单的解决方案系统。形成3D网络。通过在单个CD上制造许多这样的两腔模型，并通过旋转的离心力驱动灌注，所提出的平台提供了一种高通量和生理相关性更高的组织模型，为高成本，冗长的药物开发过程提供了简单的解决方案系统。形成3D网络。通过在单个CD上制造许多这样的两腔模型，并通过旋转的离心力驱动灌注，所提出的平台提供了一种高通量和生理相关性更高的组织模型，为高成本，冗长的药物开发过程提供了简单的解决方案系统。