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Selection and characterization of an improved strain (A-13) of Pyropia yezoensis (Bangiales, Rhodophyta)
Aquatic Botany ( IF 1.8 ) Pub Date : 2020-02-06 , DOI: 10.1016/j.aquabot.2020.103213
Hao Jiang , Hongchang Ding , Peng Zhang , Tiegan Wang , Xinghong Yan

A crossing experiment between the green mutant (C-0, ♀) and red mutant (fre, ♂) of Pyropia yezoensis was carried out for breeding an improved strain which was fast growing, high quality and wild type color, finally an improved strain (A-13) was isolated from the F1 gametophytic blades. The mean length and length-width ratio of the 60-day-old blades in A-13 were 73.43 cm and 35.41, which were 1.69 and 2.92 times higher than those of the wild-type strain (WT) of P. yezoensis, respectively, and the wet weight had no significant difference between the two strains. After being cultured for 60 days, the contents of chlorophyll a and phycobiliprotein of A-13 were 10.35 and 79.77 mg/g, which were 1.93 and 1.63 times higher than those of the WT strain, respectively. The thickness of the 60-day-old blades of A-13 strain was 19.86 μm, which was 30 % thinner than that of WT strain. In addition, the total number of conchospores per shell released from A-13 was 1408.83 × 104, which was 2.45 times higher than that of WT strain. The above results confirmed that A-13 strain was characterized by fast growth, high quality; large amount of conchospores as well as wild like color, therefore, A-13 is expected to be applied in commercial cultivation.



中文翻译:

一株改良的紫斑拟南芥(Bangiales,Rhodophyta)菌株(A-13)的选择和鉴定

进行了紫斑拟南芥绿色突变体(C-0,and)和红色突变体(fre,♂)的杂交实验,以选育出快速生长,高质量和野生型颜色的改良菌株,最终选育出改良菌株(从F 1配子体叶片中分离出A-13)。在平均长度和60日龄叶片的长度与宽度的比例A-13是73.43厘米和35.41,均较野生型菌株(高1.69和2.92倍WT的)条斑紫菜,分别,并且湿重在两个菌株之间没有显着差异。培养60天后,叶绿素含量A-13的α和藻胆蛋白分别为10.35和79.77mg / g,分别比野生型菌株高1.93和1.63倍。60天龄的A-13菌株叶片的厚度为19.86μm,比WT菌株的叶片薄30%。另外,从A-13释放的每个壳的孢子总数为1408.83×10 4,是WT菌株的2.45倍。以上结果证实A-13菌株具有生长快,品质高的特点。大量的孢子以及野生色,因此,A-13 有望用于商业化种植。

更新日期:2020-02-06
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