Myocardial Infarction (MI), also known as heart attack, is one of the most common cardiovascular diseases. Although certain drugs or mechanical means are used, day by day natural products such as herbs and spices based MI treatment is getting much popularity over the drugs or mechanical means for their pharmacological effects and have low or no side effects. This study was designed to assess the cardio-protective effect of methanolic extract of Bangladeshi multi clove garlic (Allium sativum) cultivar, a highly believed spice having cardioprotective activity, against isoproterenol (ISO) induced MI through cardiac histopathology as well as cardiac apoptotic caspase-3 gene expression study in female Wistar albino rats. Four groups containing 35 rats treated with respective agents like distill water / garlic extract (200 mg/kg-body-weight/day) up to 28 days and normal saline / ISO (100 mg/kg-body-weight/day) on 29th and 30th day were sacrificed (two rats/group/sacrifice) on the day 31, 46 and 61 and collecting the heart, cardiac histology and gene expression analysis were performed. ISO induced MI rats pretreated with garlic extract revealed up regulated expression of the cardiac apoptotic caspase-3 gene at the initial stage but finally the expressions gradually getting down regulated along with gradual improving the cardiac damage caused by apoptosis. Furthermore, only garlic extract pretreated rats were found undamaged cardioarchitecture and normal expressions of this gene. These findings suggested that garlic extract confers having significant cardioprotective effect and consuming this spice with regular diet may reduce the risk of MI.

中文翻译：

---

大蒜提取物对异丙肾上腺素诱发的大鼠心肌梗死的心脏保护作用：促凋亡caspase-3基因表达的评估

心肌梗塞（MI），也称为心脏病发作，是最常见的心血管疾病之一。尽管使用了某些药物或机械手段，但由于其药理作用，日趋天然的产品（例如基于草药和香料的MI治疗）在药物或机械手段方面越来越受欢迎，并且副作用很小或没有副作用。这项研究旨在评估孟加拉国多瓣大蒜（Allium sativum）品种甲醇提取物的心脏保护作用，这是一种具有心脏保护活性的香料，它通过心脏组织病理学以及心脏凋亡的半胱天冬酶- Wistar白化病大鼠3基因表达研究。四组包含35只大鼠，分别于29日用蒸馏水/大蒜提取物（200 mg / kg体重/天）和生理盐水/ ISO（100 mg / kg体重/天）分别处理，分别为蒸馏水/大蒜提取物（200 mg / kg体重/天）。在第31、46和61天处死小鼠和第30天（两只大鼠/组/牺牲），收集心脏，进行心脏组织学和基因表达分析。用大蒜提取物预处理的ISO诱导的MI大鼠在初始阶段显示心脏凋亡的caspase-3基因表达上调，但最终表达逐渐下调，并逐渐改善由凋亡引起的心脏损害。此外，只发现大蒜提取物预处理的大鼠的心脏结构未受损且该基因正常表达。