The multiple causes of oligohydramnios make it challenging to study. Long noncoding RNAs (lncRNAs) are sets of RNAs that have been proven to function in multiple biological processes. The purpose of this study is to study expression level and possible role of lncRNAs in oligohydramnios. In this study, total RNA was isolated from fetal membranes resected from oligohydramnios pregnant women (OP) and normal amount of amniotic fluid pregnant women (Normal). LncRNA microarray was used to analyze the differentially expressed lncRNAs and mRNAs. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to analyze the main enrichment pathways of differentially expressed mRNAs. Real-time quantitative PCR (qPCR) was used to validate the lncRNA expression level. LncRNA microarray analysis revealed that a total of 801 lncRNAs and 367 mRNAs were differentially expressed in OP; in these results, 638 lncRNAs and 189 mRNAs were upregulated, and 163 lncRNAs and 178 mRNAs were downregulated. Of the lncRNAs, 566 were intergenic lncRNAs, 351 were intronic antisense lncRNAs, and 300 were natural antisense lncRNAs. The differentially expressed lncRNAs were primarily located in chromosomes 2, 1, and 11. KEGG enrichment pathways revealed that the differentially expressed mRNAs were enriched in focal adhesion as well as in the signaling pathways of Ras, tumor necrosis factor (TNF), estrogen, and chemokine. The qPCR results confirmed that LINC00515 and RP11-388P9.2 were upregulated in OP. Furthermore, the constructed lncRNA–miRNA–mRNA regulatory network revealed tenascin R (TNR), cystic fibrosis transmembrane conductance regulator (CFTR), ATP-binding cassette sub-family A member 12 (ABCA12), and collagen 9A2 (COL9A2) as the candidate targets of LINC00515 and RP11-388P9.2. In summary, we revealed the profiles of lncRNA and mRNA in OP. These results might offer potential targets for biological prevention for pregnant women with oligohydramnios detected before delivery and provided a reliable basis for clinical biological treatment in OP.

中文翻译：

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胎膜的LncRNA和转录组分析揭示了羊水过少的潜在靶标。

羊水过少的多种原因使其难以研究。长非编码RNA（lncRNA）是已证明可在多种生物学过程中起作用的RNA集。这项研究的目的是研究羊水过少的lncRNAs的表达水平和可能的作用。在这项研究中，从羊水过少孕妇（OP）和正常羊水孕妇（正常）中切除的胎膜中分离出总RNA。用LncRNA微阵列分析差异表达的lncRNA和mRNA。京都基因与基因组百科全书（KEGG）用于分析差异表达mRNA的主要富集途径。实时定量PCR（qPCR）用于验证lncRNA表达水平。LncRNA基因芯片分析显示，OP中总共有801个lncRNA和367个mRNA差异表达。在这些结果中，638个lncRNA和189个mRNA被上调，而163个lncRNA和178个mRNA被下调。在lncRNA中，有566个是基因间的lncRNA，有351个是内含子反义lncRNA，有300个是天然反义lncRNA。差异表达的lncRNA主要位于第2、1，和11号染色体。KEGG富集途径表明，差异表达的mRNA在粘着斑以及Ras，肿瘤坏死因子（TNF），雌激素和趋化因子。qPCR结果证实LINC00515和RP11-388P9.2在OP中上调。此外，已建立的lncRNA–miRNA–mRNA调控网络揭示了腱生蛋白R（TNR），囊性纤维化跨膜电导调节剂（CFTR），ATP结合盒亚家族A成员12（ABCA12）和胶原9A2（COL9A2）作为LINC00515和RP11-388P9.2的候选靶标。总之，我们揭示了OP中lncRNA和mRNA的概况。这些结果可能为分娩前发现羊水过少的孕妇提供生物预防的潜在目标，并为OP中的临床生物治疗提供可靠的基础。