Brazilian biodiversity and favourable environmental conditions open up possibilities not yet explored, showing potential to shift the country’s monochromatic economy into an emancipated, diversified and sustainable economic environment. This can be made possible through the integral use of its resources, exploring every functional fraction to create novel solutions to modern problems. Biorefineries present an interesting strategy to fully use the potential of agricultural feedstocks and together with green separation methods can contribute to the generation of sustainable processes and products. Passion Fruit (Passiflora edulis Sims f. flavicarpa Deg species) is produced on a large scale in Brazil and in other tropical countries, and its processing plants generate tons of residues that basically consist of peel, seeds and bagasse, which account for around 75% of its mass. These fractions of P. edulis can contain significant amounts of flavonoids, secondary metabolites that are the main compounds responsible for the fruit’s bioactivity (antioxidant, anti-inflammatory, pesticide and biocide, in general). Therefore, this work aims to develop, apply and compare the best conditions for the extraction of isoorientin, orientin and isovitexin from passion fruit applying solid–liquid methodologies, followed by analyte quantification using UHPLC-PDA. Homogenizer-assisted (HAE), ultrasound-assisted (UAE) and microwave-assisted (MAE) extraction techniques were used, as well as a full factorial design to reach optimal parameters concerning the extraction yield and energy and solvent efficiencies. According to the results, the procedure based on HAE presented the best conditions for the extraction of selected flavonoids (1.07, 0.90 and 0.33 mg g−1 of isoorientin, orientin and isovitexin, respectively) and was considered the best method according to the green and sustainable described factors.

中文翻译：

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从西番莲废料中提取黄酮类化合物的绿色可持续分析方法的开发和应用。

巴西的生物多样性和有利的环境条件为尚未探索的可能性开辟了道路，显示出将该国的单色经济转变为一个解放，多样化和可持续的经济环境的潜力。通过整合利用其资源，探索每个功能部分以创建针对现代问题的新颖解决方案，这可以成为可能。生物精炼厂提出了一种有趣的策略，可以充分利用农业原料的潜力，并且与绿色分离方法一起可以促进可持续工艺和产品的产生。西番莲果（Passiflora edulis Sims f。flavicarpa Deg种）在巴西和其他热带国家大量生产，其加工厂产生大量残留物，主要由果皮，种子和蔗渣组成，约占其质量的75％。这些可食的部分可含有大量的类黄酮，次生代谢产物，它们是负责水果生物活性的主要化合物（通常是抗氧化剂，抗炎剂，杀虫剂和杀生物剂）。因此，这项工作旨在开发，应用和比较采用固液方法从百香果中提取异Orientin，Orientin和Isovitexin的最佳条件，然后使用UHPLC-PDA进行分析物定量。使用均质器辅助（HAE），超声辅助（UAE）和微波辅助（MAE）提取技术，以及全因子设计，以达到有关提取得率，能量和溶剂效率的最佳参数。根据结果​​，