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Silk fibroin nanofibrous mats for visible sensing of oxidative stress in cutaneous wounds
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-09-18 , DOI: 10.1039/d0bm01325k
Sushant Singh 1, 2, 3, 4 , Gabriela Cortes 4, 5, 6, 7 , Udit Kumar 1, 2, 3, 4 , Tamil S. Sakthivel 1, 2, 3, 4 , Stephen M. Niemiec 4, 8, 9, 10, 11 , Amanda E. Louiselle 4, 8, 9, 10, 11 , Mark Azeltine-Bannerman 4, 8, 9, 10, 11 , Carlos Zgheib 4, 8, 9, 10, 11 , Kenneth W. Liechty 4, 8, 9, 10, 11 , Sudipta Seal 1, 2, 3, 4, 12
Affiliation  

Wound healing is of major clinical concern and is constantly being explored for early restoration and enhanced recovery. While the etiology of the wound healing is multifactorial, high inflammation and increased oxidative stress which results in chronic inflammation, endothelial dysfunction and collagen degradation, delay the overall healing process. Thus, visual sensing of the oxidative stress would be highly informative in the successful implementation of wound healing therapies based on specific requirements. In this study, electrospinning was used to fabricate silk fibroin nanofibrous mats infused with Amplex red capable of detecting hydrogen peroxide, a reactive oxygen molecule. These mats produced a visible change in color with the limit of detection at 1 μM H2O2 concentration. In vivo studies carried out in diabetic mice with impaired wounds also displayed a visible change in color of the mats infused with Amplex red within 24 hours. These electrospun silk fibroin nanofibrous Amplex infused mats has the potential to enable a futuristic platform where decisions can be made for enhanced wound healing therapy.

中文翻译:

丝素蛋白纳米纤维垫,用于皮肤伤口中的氧化应激可见感测

伤口愈合是临床上最主要的问题,并且正在不断探索中,以便尽早恢复和提高恢复能力。虽然伤口愈合的病因是多因素的,但是高炎症和增加的氧化应激会导致慢性炎症,内皮功能障碍和胶原蛋白降解,从而延迟了整个愈合过程。因此,基于特定要求,视觉上的氧化应激感测在成功实施伤口愈合疗法中将是非常有用的。在这项研究中,静电纺丝被用来制造丝纤蛋白纳米纤维毡,并注入Amplex红,能够检测过氧化氢(一种活性氧分子)。这些垫在1μMH 2 O 2浓度下产生了可见的颜色变化,其检测极限。伤口受损的糖尿病小鼠中进行的体内研究还显示,在24小时内,注入Amplex红的垫子的颜色发生了明显变化。这些电纺丝素蛋白纳米纤维Amplex灌注垫具有创造未来平台的潜力,可以在该平台上做出增强伤口愈合疗法的决策。
更新日期:2020-09-25
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