Heat shock transcription factor (HSF) is ubiquitous in the whole biological world and plays an important role in regulating growth and development and responses to environment stress. In this study, a total of 60 HSF transcription factors in Brassica juncea genome were identified and analyzed. Phylogenetic analysis showed that HSF genes were divided into three groups namely: A, B, and C, of which group A was further divided into nine subgroups (A1–A9). The analysis of gene structure and conserved motifs showed that some homologous genes are highly conserved. There was strong conservative microcollinearity among Brassica rapa, B. juncea, and Brassica oleracea, which provides a basis for studying the replication of gene families. Moreover, the results revealed that the promoter regions of BjuHSF genes were rich in cis-elements related to growth and development, hormone signal, and stress response. The prediction of protein interaction results showed that HSFs could interact with multiple transcription factors and proteins in the genome, while functional annotation revealed that BjuHSF genes were involved in many biological processes. The expression patterns of BjuHSF genes were analyzed by qPCR, and the results showed that these genes were closely linked to stress response, hormones, and development process. These results are a foundation for further analysis of the regulation mechanism of HSF gene family.

中文翻译：

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芥菜中热激转录因子基因家族的全基因组分析：结构，进化和表达谱。

热激转录因子（HSF）在整个生物世界中无处不在，在调节生长和发育以及对环境压力的反应中起着重要作用。在这项研究中，鉴定并分析了芥菜基因组中总共60种HSF转录因子。系统发育分析表明，HSF基因分为三组：A，B和C，其中A组又分为9个亚组（A1-A9）。基因结构和保守基序的分析表明，一些同源基因是高度保守的。甘蓝型油菜，芥菜型油菜和甘蓝型油菜之间有很强的保守共线性。，这为研究基因家族的复制提供了基础。而且，结果表明，BjuHSF基因的启动子区域富含与生长和发育，激素信号和应激反应有关的顺式元件。对蛋白质相互作用结果的预测表明，HSFs可以与基因组中的多种转录因子和蛋白质相互作用，而功能注释则表明BjuHSF基因参与了许多生物学过程。BjuHSF的表达模式通过qPCR分析这些基因，结果表明这些基因与应激反应，激素和发育过程密切相关。这些结果为进一步分析HSF基因家族的调控机制奠定了基础。