Inferior mechanical properties have always been a limitation of the bioresorbable membranes in GBR/GTR. This study is aimed at fabricating a bioresorbable magnesium-reinforced polylactic acid- (PLA-) integrated membrane and investigating its mechanical properties, degradation rate, and biocompatibility. The uncoated and fluoride-coated magnesium alloys, AZ91, were made into strips. Then, magnesium-reinforced PLA-integrated membrane was made through integration. PLA strips were used in the control group instead of magnesium strips. Specimens were cut into rectangular shape and immersed in Hank's Balanced Salt Solution (HBSS) at 37°C for 4, 8, and 12 d. The weight loss of the AZ91 strips was measured. Three-point bending tests were conducted before and after the immersion to determine the maximum load on specimens. Potentiodynamic polarization (PDP), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) were conducted on coated and uncoated AZ91 plates to examine corrosion resistance. Murine fibroblast and osteoblast cells were cultured on circular specimens and titanium disks for 1, 3, and 5 d. Thereafter, WST test was performed to examine cell proliferation. As a result, the coated and uncoated groups showed higher maximum loads than the control group at all time points. The weight loss of AZ91 strips used in the coated group was lower than that in the uncoated group. PDP, EIS, SEM, and EDS showed that the coated AZ91 had a better corrosion resistance than the uncoated AZ91. The cell proliferation test showed that the addition of AZ91 did not have an adverse effect on osteoblast cells. Conclusively, the magnesium-reinforced PLA-integrated membrane has excellent load capacity, corrosion resistance, cell affinity, and proper degradation rate. Moreover, it has great potential as a bioresorbable membrane in the GBR/GTR application.

中文翻译：

---

作为牙科应用的生物可吸收镁增强 PLA 集成 GTR/GBR 膜的表征

较差的机械性能一直是 GBR/GTR 中生物可吸收膜的局限性。本研究旨在制造可生物吸收的镁增强聚乳酸（PLA-）集成膜并研究其机械性能、降解速率和生物相容性。无涂层和氟化物涂层的镁合金 AZ91 被制成带材。然后，通过集成制成镁增强PLA集成膜。对照组使用PLA条代替镁条。将标本切成长方形，在 37°C 的汉克平衡盐溶液 (HBSS) 中浸泡 4、8 和 12 天。测量了 AZ91 条的重量损失。在浸入前后进行三点弯曲试验以确定试样的最大载荷。动电位极化 (PDP)、电化学阻抗谱 (EIS)、扫描电子显微镜 (SEM) 和能量色散谱 (EDS) 在涂层和未涂层​​ AZ91 板上进行，以检查耐腐蚀性。鼠成纤维细胞和成骨细胞在圆形标本和钛盘上培养 1、3 和 5 天。此后，进行WST测试以检查细胞增殖。结果，涂层和未涂层​​组在所有时间点都显示出比对照组更高的最大负荷。涂层组使用的AZ91条的失重低于未涂层组。PDP、EIS、SEM 和 EDS 表明，带涂层的 AZ91 比未涂层的 AZ91 具有更好的耐腐蚀性。细胞增殖试验表明AZ91的加入对成骨细胞没有不良影响。总之，镁增强 PLA 集成膜具有出色的负载能力、耐腐蚀性、细胞亲和力和适当的降解率。此外，它在 GBR/GTR 应用中作为生物可吸收膜具有巨大潜力。