An effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in controlling the proliferation of mesenchymal stem cells (MSCs) remain to be understood. This study investigated the regulation of transient quiescence using growth curves, proliferation assay, the cytometric evaluation of cell cycle, Western blotting, and the electromobility gel shift assay (EMSA) on synchronized MSCs of the C3H10Т1/2 and control cells with different statuses of pRb proteins. It has been found that functional steady-state level of p130 but not pRb plays a critical role for entering, exiting, and maintenance of transient quiescence in multipotent mesenchymal stem cells.

中文翻译：

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p130和pRb维持间充质干细胞瞬时静止

静态的有效调节在干细胞的分化，可塑性和防止干细胞变恶性中起关键作用。静止状态受pRb家族蛋白的控制，这些蛋白在细胞周期调控中显示出重叠的功能。然而，它们在控制间充质干细胞（MSCs）增殖中的作用仍有待了解。本研究使用生长曲线，增殖测定，细胞周期的细胞计数评估，Western印迹和电动迁移凝胶位移测定（EMSA）对C3H10Т1/ 2同步MSC和不同pRb状态的对照细胞进行了瞬时静止的调节蛋白质。已经发现，p130的功能稳态水平而非pRb的稳态水平对于进入，退出，