Synthetic DNA has recently risen as a viable alternative for long-term digital data storage. To ensure that information is safely recovered after storage, it is essential to appropriately preserve the physical DNA molecules encoding the data. While preservation of biological DNA has been studied previously, synthetic DNA differs in that it is typically much shorter in length, it has different sequence profiles with fewer, if any, repeats (or homopolymers), and it has different contaminants. In this paper we evaluate nine different methods used to preserve data files encoded in synthetic DNA by accelerated aging of nearly 29,000 DNA sequences. In addition to a molecular count comparison, we also sequence and analyze the DNA after aging. Our findings show that errors and erasures are stochastic and show no practical distribution difference between preservation methods. Finally, we compare the physical density of these methods and provide a stability versus density trade-offs discussion.

中文翻译：

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合成DNA数据保存方法的经验比较

合成DNA最近已成为长期数字数据存储的可行替代方案。为了确保在存储后可以安全地恢复信息，必须适当保存编码数据的物理DNA分子。虽然先前已经研究了生物DNA的保存，但合成DNA的不同之处在于其长度通常要短得多，具有不同的序列特征，重复次数（或均聚物）更少（如果有的话），并且具有不同的污染物。在本文中，我们评估了通过加速近29,000个DNA序列的老化来保存合成DNA编码的数据文件的9种不同方法。除了进行分子计数比较外，我们还对老化后的DNA进行测序和分析。我们的发现表明，错误和擦除是随机的，并且在保存方法之间没有实际的分配差异。最后，我们比较了这些方法的物理密度，并提供了稳定性与密度权衡的讨论。