The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson’s disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).

中文翻译：

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帕金森氏病诊断中神经元外泌体标志物的简便阻抗分析。

神经元来源的外泌体中α-突触核蛋白的释放早于帕金森氏病（PD）的临床表现，为开展预测性或预后性测试提供了一种手段。在这里，我们报告了来自神经外泌体的两个内部外泌体标记物（α-突触核蛋白和合成蛋白-1）的无试剂阻抗测定。在裂解之前，使用抗L1CAM共轭的两性离子聚合物修饰的磁珠从患者血清中有效提取外泌体，并通过电化学阻抗谱进行分析。在40个临床样品中对α-突触核蛋白水平进行定量分析，可以解决PD患者与健康对照（HC）之间的统计学差异。