The induced dwarf mutant Rht12 was previously shown to have agronomic potential to replace the conventional DELLA mutants Rht-B1b/Rht-D1b in wheat. The Rht12 dwarfing gene is not associated with reduced coleoptile length (unlike the DELLA mutants) and it is dominant, characteristics which are shared with the previously characterized dwarfing genes Rht18 and Rht14. Using the Rht18/Rht14 model, a gibberellin (GA) 2-oxidase gene was identified in the Rht12 region on chromosome 5A. A screen for suppressor mutants in the Rht12 background identified tall overgrowth individuals that were shown to contain loss-of-function mutations in GA2oxidaseA13, demonstrating the role of this gene in the Rht12 dwarf phenotype. It was concluded that Rht12, Rht18, and Rht14 share the same height-reducing mechanism through the increased expression of GA 2-oxidase genes. Some of the overgrowth mutants generated in this study were semi-dwarf and taller than the original Rht12 dwarf, providing breeders with new sources of agronomically useful dwarfism.

中文翻译：

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过度生长的突变体决定了GA2氧化酶A13在小麦Rht12矮化中的因果作用。

先前显示诱导的矮化突变体Rht12具有替代小麦中常规DELLA突变体Rht-B1b / Rht-D1b的农艺潜力。所述Rht12矮化基因不具有降低的胚芽鞘长度（不像DELLA突变体）相关联，并且它是占主导地位的，这是与以前表征的矮化基因共享特征Rht18和Rht14。使用Rht18 / Rht14模型，在5A染色体的Rht12区鉴定出了赤霉素（GA）2-氧化酶基因。在Rht12背景中进行抑制突变体筛选时，发现了高个子的过度生长个体，这些个体被证明含有功能丧失突变。GA2oxidaseA13，证明该基因在Rht12矮表型中的作用。结论是，通过增加GA 2-氧化酶基因的表达，Rht12，Rht18和Rht14具有相同的高度降低机制。在这项研究中产生的一些过度生长的突变体是半矮的，并且比原始的Rht12矮高，为育种者提供了农业上有用的矮化的新来源。