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EMILIN proteins are novel extracellular constituents of the dentin-pulp complex.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-09-18 , DOI: 10.1038/s41598-020-72123-2
Thomas Imhof 1, 2 , Yüksel Korkmaz 2, 3 , Manuel Koch 1, 2 , Gerhard Sengle 1, 4, 5, 6 , Alvise Schiavinato 1
Affiliation  

Odontoblasts and pulp stroma cells are embedded within supramolecular networks of extracellular matrix (ECM). Fibrillin microfibrils and associated proteins are crucial constituents of these networks, serving as contextual scaffolds to regulate tissue development and homeostasis by providing both structural and mechanical properties and sequestering growth factors of the TGF-β superfamily. EMILIN-1, -2, and -3 are microfibril-associated glycoproteins known to modulate cell behaviour, growth factor activity, and ECM assembly. So far their expression in the various cells of the dentin-pulp complex during development, in the adult stage, and during inflammation has not been investigated. Confocal immunofluorescence microscopy and western blot analysis of developing and adult mouse molars and incisors revealed an abundant presence of EMILINs in the entire dental papilla, at early developmental stages. Later in development the signal intensity for EMILIN-3 decreases, while EMILIN-1 and -2 staining appears to increase in the pre-dentin and in the ECM surrounding odontoblasts. Our data also demonstrate new specific interactions of EMILINs with fibulins in the dentin enamel junction. Interestingly, in dentin caries lesions the signal for EMILIN-3 was significantly increased in inflamed odontoblasts. Overall our findings point for the first time to a role of EMILINs in dentinogenesis, pulp biology, and inflammation.



中文翻译:

EMILIN 蛋白是牙本质-牙髓复合体的新型细胞外成分。

成牙本质细胞和牙髓基质细胞嵌入细胞外基质 (ECM) 的超分子网络中。原纤维蛋白微原纤维和相关蛋白质是这些网络的重要组成部分,作为背景支架,通过提供结构和机械特性以及隔离 TGF-β 超家族的生长因子来调节组织发育和体内平衡。EMILIN-1、-2 和 -3 是已知可调节细胞行为、生长因子活性和 ECM 组装的微纤维相关糖蛋白。迄今为止,尚未研究它们在发育、成年期和炎症期间在牙本质-牙髓复合体的各种细胞中的表达。发育中和成年小鼠磨牙和门牙的共聚焦免疫荧光显微镜和蛋白质印迹分析显示,在早期发育阶段,整个牙乳头中大量存在 EMILIN。在发育后期,EMILIN-3 的信号强度降低,而 EMILIN-1 和 -2 染色在牙本质前体和成牙本质细胞周围的 ECM 中似乎增加。我们的数据还证明了 EMILINs 与牙本质釉质连接处的纤维蛋白的新的特异性相互作用。有趣的是,在牙本质龋损中,发炎的成牙本质细胞中 EMILIN-3 的信号显着增加。总的来说,我们的发现首次指出 EMILIN 在牙本质发生、牙髓生物学和炎症中的作用。而 EMILIN-1 和 -2 染色似乎在牙本质前体和成牙本质细胞周围的 ECM 中增加。我们的数据还证明了 EMILINs 与牙本质釉质连接处的纤维蛋白的新的特异性相互作用。有趣的是,在牙本质龋损中,发炎的成牙本质细胞中 EMILIN-3 的信号显着增加。总的来说,我们的发现首次指出 EMILIN 在牙本质发生、牙髓生物学和炎症中的作用。而 EMILIN-1 和 -2 染色似乎在牙本质前体和成牙本质细胞周围的 ECM 中增加。我们的数据还证明了 EMILINs 与牙本质釉质连接处的纤维蛋白的新的特异性相互作用。有趣的是,在牙本质龋损中,发炎的成牙本质细胞中 EMILIN-3 的信号显着增加。总的来说,我们的发现首次指出 EMILIN 在牙本质发生、牙髓生物学和炎症中的作用。

更新日期:2020-09-20
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