ABSTRACT Animals developed or in an embryonic stage, are constantly subjected to magnetic pollution generated by electrical and electronic devices. Several researches have used the bird embryo as an experimental model to evaluate the action of magnetic field (MF) and electromagnetic field (EMF). This study proposed to perform a morphometric evaluation in the embryos and in the blood vascular network of the yolk sac membranes (YSM) of Japanese quail (Coturnix japonica) exposed to the 60 Hz MF with two different intensities (0.16 and 0.65 mT). A total of 30 eggs were used, 10 eggs were used for each assay. Each assay formed a group (control group, group submitted to the MF of 0.16 mT and 0.65 mT). The images of the skeletonized vascular network of YSM were evaluated by two methods of fractal dimension: box-counting dimension (Dbc) and information dimension (Dinf). The embryos were evaluated by body mass, percentage cephalic length and body area. The fractal dimensions revealed no difference among groups. There were no significant differences in relation to embryonic body mass among groups. However, the embryos exposed to 0.65 mT MF presented a smaller embryonic body development (body area and percentage cephalic length). In conclusion, 0.16 mT and 0.65 mT magnetic fields were not able to generate significant effects on vasculogenesis and angiogenesis. However, the embryos exposed to 6 h of magnetic field with 0.65 mT intensity and 60 Hz frequency showed a decrease in embryonic body development.

中文翻译：

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两种不同强度低频磁场对日本鹌鹑胚胎及其胚外血管网络的形态测量评价

摘要 发育或处于胚胎阶段的动物不断受到电气和电子设备产生的磁污染。多项研究已使用鸟类胚胎作为实验模型来评估磁场 (MF) 和电磁场 (EMF) 的作用。本研究提议对暴露于两种不同强度（0.16 和 0.65 mT）的 60 Hz MF 的日本鹌鹑（Coturnix japonica）的胚胎和卵黄囊膜 (YSM) 的血管网络进行形态测量评估。总共使用30个鸡蛋，每次测定使用10个鸡蛋。每个测定形成一个组（对照组，接受 0.16 mT 和 0.65 mT MF 的组）。YSM的骨架化血管网络的图像通过分形维数的两种方法进行评估：box-counting 维度 (Dbc) 和信息维度 (Dinf)。胚胎通过体重、头部长度百分比和身体面积进行评估。分形维数显示组间没有差异。各组之间的胚胎体重没有显着差异。然而，暴露于 0.65 mT MF 的胚胎呈现出较小的胚胎体发育（体面积和头部长度百分比）。总之，0.16 mT 和 0.65 mT 磁场不能对血管生成和血管生成产生显着影响。然而，暴露于 0.65 mT 强度和 60 Hz 频率的磁场 6 小时的胚胎显示胚胎体发育下降。分形维数显示组间没有差异。各组之间的胚胎体重没有显着差异。然而，暴露于 0.65 mT MF 的胚胎呈现出较小的胚胎体发育（体面积和头部长度百分比）。总之，0.16 mT 和 0.65 mT 磁场不能对血管生成和血管生成产生显着影响。然而，暴露于 0.65 mT 强度和 60 Hz 频率的磁场 6 小时的胚胎显示胚胎体发育下降。分形维数显示组间没有差异。各组之间的胚胎体重没有显着差异。然而，暴露于 0.65 mT MF 的胚胎呈现出较小的胚胎体发育（体面积和头部长度百分比）。总之，0.16 mT 和 0.65 mT 磁场不能对血管生成和血管生成产生显着影响。然而，暴露于 0.65 mT 强度和 60 Hz 频率的磁场 6 小时的胚胎显示胚胎体发育下降。65 mT 磁场不能对血管生成和血管生成产生显着影响。然而，暴露于 0.65 mT 强度和 60 Hz 频率的磁场 6 小时的胚胎显示胚胎体发育下降。65 mT 磁场不能对血管生成和血管生成产生显着影响。然而，暴露于 0.65 mT 强度和 60 Hz 频率的磁场 6 小时的胚胎显示胚胎体发育下降。