Abstract

The transgenic soybean expressing the cry1Ac insecticidal gene of the Bacillus thuringiensis bacterium has been widely adopted in agriculture. This bacterium acts as an efficient control agent of pest insects due to the expression of different proteins with entomopathogenic activity such as Cry and Vip. This study investigates the toxicity and mode of interaction between the Cry1Ab, Cry1Ac, Cry2Aa, Cry2Ab and Vip3Aa proteins against Chrysodeixis includens and Anticarsia gemmatalis larvae. The combination of the Cry1Ac, Cry2Aa and Vip3Aa toxins had a synergistic effect for C. includens, however, only Vip3Aa and Cry2Aa exhibited synergistic interaction against A. gemmatalis. Cry1Ab and Cry1Ac proteins have been shown to share receptors with the two species tested, however, Cry1Ac does not share binding sites with Cry2Aa, Cry2Ab, and Vip3Aa in both species. Besides helping the resistance management to B. thuringiensis proteins, the combination of Cry and Vip proteins can increase toxicity through synergistic action.

摘要

表达苏云金芽孢杆菌cry1Ac杀虫基因的转基因大豆已广泛应用于农业。由于表达具有昆虫病原活性的不同蛋白质，如 Cry 和 Vip，这种细菌可作为害虫的有效控制剂。本研究调查了 Cry1Ab、Cry1Ac、Cry2Aa、Cry2Ab 和 Vip3Aa 蛋白对Chrysodeixis includens和Anticarsia gemmatalis幼虫的毒性和相互作用模式。Cry1Ac、Cry2Aa 和 Vip3Aa 毒素的组合对C. includens具有协同作用，然而，只有 Vip3Aa 和 Cry2Aa 对A. gemmatalis表现出协同作用. Cry1Ab 和 Cry1Ac 蛋白已被证明与所测试的两个物种共享受体，但是，Cry1Ac 在这两个物种中不与 Cry2Aa、Cry2Ab 和 Vip3Aa 共享结合位点。除了帮助对B. thuringiensis蛋白的抗性管理外，Cry 和 Vip 蛋白的组合还可以通过协同作用增加毒性。