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Abdominal angiostrongyliasis can be diagnosed with a immunochromatographic rapid test with recombinant galactin from Angiostrongylus cantonensis.
Memórias do Instituto Oswaldo Cruz ( IF 2.8 ) Pub Date : 2020-09-18 , DOI: 10.1590/0074-02760200201
Carlos Graeff-Teixeira 1 , Vanessa Fey Pascoal 2 , Rubens Rodriguez 3 , Alessandra Loureiro Morassutti 3 , Pewpan M Intapan 4 , Wanchai Maleewong 4
Affiliation  

Angiostrongylus costaricensis is the causative agent of abdominal angiostrongyliasis, a zoonotic infection that may produce severe eosinophilic enterocolitis or hepatitis in humans. Parasites are usually not released in stools and serology has an important role in diagnosis. Since cross-reactivity is demonstrated between A. costaricensis and another metastrongylid worm, A. cantonensis, we tested heterologous recombinant galectin as a probe in an immunochromatographic rapid diagnostic test (ICT-RDT) for detection of anti-A. costaricensis antibodies. Almost all (11/12) positive control sera from A. costaricensis infected patients were positive at ICT RDT. These are preliminary indications that r-galectin ICT-RDT is useful for diagnosing A. costaricensis infection.

中文翻译:

可以使用来自广州血管圆线虫的重组半乳糖蛋白进行免疫色谱快速检测,诊断出腹腔血管圆环霉菌病。

肋脉管圆线虫是腹部血管圆斑病的病原体,这种疾病是一种人畜共患的感染,可能在人体内引起严重的嗜酸性小肠结肠炎或肝炎。寄生虫通常不会在粪便中释放,血清学在诊断中具有重要作用。由于证明了A.costaricensis和另一种拟圆虫蠕虫A.cantonensis之间的交叉反应性,我们在免疫色谱快速诊断测试(ICT-RDT)中检测了异源重组半乳糖凝集素作为探针来检测抗A。椰子抗体。几乎所有(11/12)阳性阳性对照血清均来自A. costaricensis感染患者,ICT RDT阳性。这些初步迹象表明,r-半乳糖凝集素ICT-RDT可用于诊断哥斯达黎加感染。
更新日期:2020-09-20
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