Oocyte maturation plays a vitally important role in porcine reproduction. Regrettably, the quality of oocytes matured in vitro is weaker than that of in vivo matured oocytes. We collected and cultivated porcine cumulus oocyte complexes (COCs) in vitro with phosphoinositide‐dependent kinase 1 (PDK1) activator 5‐(4‐chloro‐phenyl)‐3‐phenyl‐pent‐2‐enoic acid (PS48), whose concentrations were 0, 2, 5, 10 and 20 µM to investigate whether the phosphatidylinositol‐3‐kinase/protein kinase B (PI3K/Akt) signalling pathway would impact the oocyte quality. The results showed that 10 µM PS48 increased the oocyte proportion of metaphase II (MII) stage and improved the expansion of cumulus cells (CCs). What's more, the activation of PI3K/Akt signalling pathway could regulate the expression of maturation‐related genes and proteins. The results of quantitative real‐time PCR showed that 10 µM PS48 increased the mRNA and protein levels of Akt and regulated maturation‐related genes, including cyclin B1, MOS, BMP15, GDF9, CDC2, mTOR, BAX, BCL2 and caspase‐3. The results of Western blot indicated that 10µM PS48 increased the protein abundance of Akt, phosphorylation of Akt Thr308 (p‐Akt^Thr308) and cyclin B1, but decreased the protein abundance of pro‐apoptotic BAX. These results suggested that adding 10 µM PS48 to mature culture medium could promote the maturation of porcine oocytes, potentially through activating the PI3K/Akt signalling pathway.

中文翻译：

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PS48通过激活PI3K / Akt信号通路促进猪卵母细胞的体外成熟和发育能力。

卵母细胞的成熟在猪的繁殖中起着至关重要的作用。遗憾的是，体外成熟的卵母细胞的质量比体内成熟的卵母细胞的质量弱。我们使用磷酸肌醇依赖性激酶1（PDK1）活化剂5-（4-氯-苯基）-3-苯基-戊基-2-烯酸（PS48）体外收集和培养了猪卵丘卵母细胞复合物（COC）。 0、2、5、10和20 µM，以研究磷脂酰肌醇3-激酶/蛋白激酶B（PI3K / Akt）信号通路是否会影响卵母细胞质量。结果显示，10 µM PS48可增加中期II（MII）期的卵母细胞比例，并改善卵丘细胞（CCs）的扩增。此外，PI3K / Akt信号通路的激活可以调节与成熟相关的基因和蛋白质的表达。细胞周期蛋白B1，MOS，BMP15，GDF9，CDC2，mTOR，BAX，BCL2和caspase-3。Western blot结果表明，10μMPS48可增加Akt的蛋白质丰度，Akt Thr308（p‐Akt ^Thr308）和细胞周期蛋白B1的磷酸化，但降低促凋亡BAX的蛋白质丰度。这些结果表明，向成熟培养基中添加10 µM PS48可以通过激活PI3K / Akt信号通路来促进猪卵母细胞的成熟。