Identification of the molecular target is a crucial step in evaluating novel antibiotics. To support target identification, a label‐free method based on chromatographic co‐elution has previously been developed. Target identification by chromatographic coelution (TICC) exploits the alteration of the elution profile of target‐bound drug versus free drug in ion exchange (IEX) chromatography to identify potential target proteins from elution fractions. The applicability of TICC for antibiotic research is investigated by evaluating which proteins, that is, putative targets, can be monitored in Bacillus subtilis. Coelution of components of known protein complexes provides a read‐out for how well the native state of proteins is conserved during chromatography. Rifampicin, which targets RNA polymerase, is used in a proof‐of‐concept study.

中文翻译：

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色谱共洗脱在抗生素靶标鉴定中的适用性。

分子靶点的鉴定是评价新型抗生素的关键步骤。为了支持目标识别，之前已经开发了一种基于色谱共洗脱的无标记方法。通过色谱共流出 (TICC) 进行靶标识别利用离子交换 (IEX) 色谱中靶标结合药物与游离药物的洗脱曲线的变化来识别洗脱级分中的潜在靶蛋白。通过评估可以在枯草芽孢杆菌中监测哪些蛋白质（即推定目标）来研究 TICC 在抗生素研究中的适用性. 已知蛋白质复合物组分的共流出提供了在色谱过程中蛋白质天然状态保存程度的读数。以 RNA 聚合酶为靶点的利福平用于概念验证研究。