Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four‐stranded β‐sheet that is flanked by four α‐helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.

中文翻译：

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Se0862 的溶液 NMR 结构，Se0862 是一种参与生物膜形成的高度保守的蓝藻蛋白。


生物膜是嵌入细胞外基质中的微生物的积累，可以抵御外部因素和应激环境。蓝藻生物膜无处不在，具有废水处理和生物燃料可持续生产的潜力。但调节蓝藻生物膜形成的潜在机制尚不清楚。在这里，我们报告了蛋白质 Se0862 的溶液 NMR 结构，该蛋白质在不同的蓝藻物种中保守，并参与蓝藻细长聚球藻PCC 7942 中生物膜形成的调节。Se0862 是一种 α+β 类蛋白质，具有 ααββββαα 拓扑和卷结构，由一侧有四个 α 螺旋的四链 β 折叠。保守的表面残基构成疏水袋和带电区域，这些区域可能也存在于 Se0862 直系同源物中。