A combination of bioceramics and nanofibrous scaffolds holds promising potential for inducing of mineralization in connective tissues. The aim of the present study was to investigate the attachment, proliferation and odontogenic differentiation of dental pulp stem cells (DPSC) on poly(l‐lactide) (PLLA) nanofibers coated with mineral trioxide aggregate (MTA). Polymeric scaffolds were fabricated via the electrospinning method and their surface was coated with MTA. DPSC were isolated from dental pulp and their biological behavior was evaluated on scaffolds and the control group using MTT assay. Alkaline phosphatase (ALP) activity, biomineralization and the expression of odontogenic genes were analyzed during odontogenic differentiation. Isolated DPSC showed spindle‐shaped morphology with multi‐lineage differentiation potential and were positive for CD73, CD90 and CD105. MTA‐coated PLLA (PLLA/MTA) exhibited nanofibrous structure with average fiber diameter of 756 ± 157 nm and interconnected pores and also suitable mechanical properties. Similar to MTA, these scaffolds were shown to be biocompatible and to support the attachment and proliferation of DPSC. ALP activity transiently peaked on day 14 and was significantly higher in PLLA/MTA scaffolds than in the control groups. In addition, increasing biomineralization was observed in all groups with a higher amount in PLLA/MTA. Odontogenic‐related genes, DSPP and collagen type I showed a higher expression in PLLA/MTA on days 21 and 14, respectively. Taken together, MTA/PLLA electrospun nanofibers enhanced the odontogenic differentiation of DPSC and showed the desired characteristics of a pulp capping material.

中文翻译：

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包覆有三氧化二矿物聚集体的电纺聚（丙交酯）纳米纤维可增强牙髓干细胞的牙源性分化

生物陶瓷和纳米纤维支架的组合具有诱人的潜力，可在结缔组织中诱导矿化。本研究的目的是研究牙髓干细胞（DPSC）在聚（l）上的附着，增殖和成牙分化。-丙交酯（PLLA）纳米纤维涂有三氧化二无机骨料（MTA）。聚合物支架通过静电纺丝法制备，其表面涂有MTA。从牙髓中分离DPSC，并使用MTT分析法在支架和对照组上评估其生物学行为。在牙源性分化过程中分析了碱性磷酸酶（ALP）的活性，生物矿化和牙源性基因的表达。分离的DPSC显示纺锤状形态，具有多谱系分化潜能，并且对CD73，CD90和CD105呈阳性。MTA涂层的PLLA（PLLA / MTA）表现出纳米纤维结构，平均纤维直径为756±157 nm，具有相互连接的孔以及适当的机械性能。与MTA类似，这些支架显示出生物相容性，并支持DPSC的附着和增殖。ALP活性在第14天短暂达到峰值，并且在PLLA / MTA支架中明显高于对照组。另外，在所有组中观察到生物矿化的增加，并且PLLA / MTA的含量更高。牙源性相关基因，DSPP和I型胶原分别在第21天和第14天在PLLA / MTA中表达较高。两者合计，MTA / PLLA电纺纳米纤维增强了DPSC的牙源性分化，并显示了纸浆覆盖材料的所需特性。在PLLA / MTA含量较高的所有组中均观察到生物矿化增加。牙源性相关基因，DSPP和I型胶原分别在第21天和第14天在PLLA / MTA中表达较高。两者合计，MTA / PLLA电纺纳米纤维增强了DPSC的牙源性分化，并显示了纸浆覆盖材料的所需特性。在PLLA / MTA含量较高的所有组中均观察到生物矿化增加。牙源性相关基因，DSPP和I型胶原分别在第21天和第14天在PLLA / MTA中表达较高。两者合计，MTA / PLLA电纺纳米纤维增强了DPSC的牙源性分化，并显示了纸浆覆盖材料的所需特性。