Surface‐enhanced Raman scattering of secretory proteins for the cytotoxicity analysis of low‐dose doxorubicin,Journal of Raman Spectroscopy

当前位置： X-MOL 学术 › J. Raman Spectrosc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Surface‐enhanced Raman scattering of secretory proteins for the cytotoxicity analysis of low‐dose doxorubicin
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2020-09-19 , DOI: 10.1002/jrs.5990
Mengmeng Zheng ₁ , Siqi Gao ₁ , Yamin Lin ₁ , Yating Lin ₁ , Zufang Huang ₁ , Shusen Xie ₁ , Yun Yu ₂ , Juqiang Lin ₁

Affiliation

The cytotoxicity and dose of anticancer drugs must be strictly controlled to achieve better therapeutic effects while reducing side effects. Here, surface‐enhanced Raman scattering (SERS) of secretory proteins was employed to analyze the cytotoxicity of doxorubicin at low dose (0.01 and 0.05 μg/ml), which could not be evaluated by the conventional methyl thiazolyl blue tetrazolium bromide (MTT) assay. The variances of SERS signals of secretory proteins between control and doxorubicin‐treated groups became greater gradually with the increase of doxorubicin dosage, and the SERS bands at 660 and 1,375 cm⁻¹ were closely related to the cytotoxicity of doxorubicin. Combined with principal component analysis and linear discriminant analysis (PCA‐LDA), SERS spectra of secretory proteins from different drug dose groups could be distinguished with high sensitivity (97.2%) and accuracy (84.7%), demonstrating promising potential as an alternative nanotechnology for cytotoxicity analysis of low‐dose anticancer drugs.

中文翻译：

分泌蛋白的表面增强拉曼散射对低剂量阿霉素的细胞毒性分析

必须严格控制抗癌药物的细胞毒性和剂量，以达到更好的治疗效果，同时减少副作用。在这里，分泌蛋白的表面增强拉曼散射（SERS）用于分析低剂量（0.01和0.05μg/ ml）时阿霉素的细胞毒性，而传统的甲基噻唑基蓝四唑溴化物（MTT）分析无法评估阿霉素的细胞毒性。。对照组和阿霉素处理组之间分泌蛋白的SERS信号方差随着阿霉素剂量的增加而逐渐增大，SERS谱带在660和1,375 cm ^-1处与阿霉素的细胞毒性密切相关。结合主成分分析和线性判别分析（PCA-LDA），可以高灵敏度（97.2％）和准确度（84.7％）区分不同药物剂量组的分泌蛋白的SERS光谱，证明了其作为替代纳米技术的潜力低剂量抗癌药的细胞毒性分析。

更新日期：2020-11-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11