Zinc supplementation alleviates endoplasmic reticulum stress during porcine oocyte in vitro maturation by upregulating zinc transporters,Journal of Cellular Physiology

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Zinc supplementation alleviates endoplasmic reticulum stress during porcine oocyte in vitro maturation by upregulating zinc transporters
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-09-17 , DOI: 10.1002/jcp.30052
Muhammad Rosyid Ridlo _{1,

2} , Geon A Kim _{1,

3} , Anukul Taweechaipaisankul ₁ , Eui Hyun Kim ₁ , Byeong Chun Lee ₁

Affiliation

Endoplasmic reticulum (ER) stress is a major contributor to embryonic development failure. Mammalian oocytes have a high risk of exposure to cellular stress during in vitro embryo production. We investigated the effects of zinc supplementation during in vitro maturation under ER stress. We evaluated cumulus expansion, embryonic development derived by parthenogenetic activation, reactive oxygen species, protein expression of X‐box binding protein 1 (XBP1), and expression of genes related to ER stress. Supplementation with 1 μg/ml zinc significantly increased the nuclear maturation of oocytes, cleavage and blastocyst formation rates, and total blastocyst cell number (p < .05). Under ER stress, zinc significantly reduced protein expression of XBP1, and increased cleavage and blastocyst rates (p < .05). Concomitantly, zinc supplementation upregulated the expression of zinc transporters (SLC39A14 and SLC39A10), PTGS2, and downregulated ER stress‐related genes (sXBP1, uXBP1, ATF4, and PTPN1/PTP1B), and caspase 3. These results suggest that zinc supplementation alleviates ER stress by providing essential metal‐ion transporters for oocyte maturation and subsequent embryonic development.

中文翻译：

补锌通过上调锌转运蛋白减轻猪卵母细胞体外成熟过程中的内质网应激

内质网 (ER) 应激是胚胎发育失败的主要因素。哺乳动物卵母细胞在体外胚胎生产过程中暴露于细胞应激的风险很高。我们研究了在 ER 应激下的体外成熟过程中补充锌的影响。我们评估了卵丘扩张、孤雌生殖激活衍生的胚胎发育、活性氧、X-box 结合蛋白 1 (XBP1) 的蛋白质表达以及与内质网应激相关基因的表达。补充 1 μg/ml 锌可显着增加卵母细胞的核成熟、卵裂和囊胚形成率以及总囊胚细胞数 ( p < .05)。ER应激下，锌显着降低XBP1蛋白表达, 并增加卵裂和囊胚率 ( p < .05)。同时，补锌上调锌转运蛋白（SLC39A14和SLC39A10）、PTGS2和下调内质网应激相关基因（sXBP1、uXBP1、ATF4和PTPN1/PTP1B）和caspase 3 的表达。这些结果表明，锌补充剂通过为卵母细胞成熟和随后的胚胎发育提供必需的金属离子转运蛋白来减轻内质网应激。

更新日期：2020-09-17

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