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Wolbachia affects reproduction in the spider mite Tetranychus truncatus (Acari: Tetranychidae) by regulating chorion protein S38-like and Rop.
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-09-18 , DOI: 10.1111/imb.12669 X Xia 1 , C-W Peng 1 , J-R Cui 1 , P-Y Jin 1 , K Yang 1 , X-Y Hong 1
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-09-18 , DOI: 10.1111/imb.12669 X Xia 1 , C-W Peng 1 , J-R Cui 1 , P-Y Jin 1 , K Yang 1 , X-Y Hong 1
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Wolbachia‐induced reproductive regulation in hosts has been used to control pest populations, but little is known about the molecular mechanism underlying Wolbachia regulation of host genes. Here, reproductive regulation by Wolbachia in the spider mite Tetranychus truncatus was studied at the molecular level. Infection with Wolbachia resulted in decreasing oviposition and cytoplasmic incompatibility in T. truncatus. Further RNA‐seq revealed genes regulated by Wolbachia in T. truncatus. Real‐time quantitative polymerase chain reaction (qPCR) showed that genes, including chorion protein S38‐like and Rop were down‐regulated by Wolbachia. RNA interference (RNAi) of chorion protein S38‐like and Rop in Wolbachia‐uninfected T. truncatus decreased oviposition, which was consistent with Wolbachia‐induced oviposition decrease. Interestingly, suppressing Rop in Wolbachia‐infected T. truncatus led to increased Wolbachia titres in eggs; however, this did not occur after RNAi of chorion protein S38‐like. This is the first study to show that chorion protein S38‐like and Rop facilitate Wolbachia‐mediated changes in T. truncatus fertility. In addition, RNAi of Rop turned the body colour of Wolbachia‐uninfected T. truncatus black, which indicates that the role of Rop is not limited to the reproductive regulation of T. truncatus.
中文翻译：
Wolbachia 通过调节绒毛膜蛋白 S38 样和 Rop 影响叶螨 Tetranychus truncatus（Acari：Tetranychidae）的繁殖。
Wolbachia诱导宿主的生殖调控已被用于控制害虫种群，但对Wolbachia调控宿主基因的分子机制知之甚少。在这里，在分子水平上研究了Wolbachia在叶螨Tetranychus truncatus中的生殖调节。感染Wolbachia导致T. truncatus 的产卵减少和细胞质不相容性。进一步的 RNA-seq 揭示了T. truncatus 中受Wolbachia调控的基因。实时定量聚合酶链反应 (qPCR) 显示基因，包括绒毛膜蛋白 S38 样和Rop被Wolbachia下调。的RNA干扰（RNAi）样S38绒毛膜蛋白和罗普在Wolbachia-未感染T.截产卵下降，这与一致的沃尔巴克氏体诱导的产卵减少。有趣的是，在Wolbachia感染的T. truncatus 中抑制Rop导致鸡蛋中Wolbachia滴度增加；然而，在绒毛膜蛋白 S38 样的RNAi 之后，这并没有发生。这是第一项表明绒毛膜蛋白 S38 样和Rop促进Wolbachia-T. truncatus生育力的介导变化。此外，RNA干扰罗普转向的车身颜色沃尔巴克氏体-uninfected T.截黑色，这表明的作用罗普不限于生殖调控T.截。
更新日期：2020-09-18
中文翻译：
Wolbachia 通过调节绒毛膜蛋白 S38 样和 Rop 影响叶螨 Tetranychus truncatus（Acari：Tetranychidae）的繁殖。
Wolbachia诱导宿主的生殖调控已被用于控制害虫种群，但对Wolbachia调控宿主基因的分子机制知之甚少。在这里，在分子水平上研究了Wolbachia在叶螨Tetranychus truncatus中的生殖调节。感染Wolbachia导致T. truncatus 的产卵减少和细胞质不相容性。进一步的 RNA-seq 揭示了T. truncatus 中受Wolbachia调控的基因。实时定量聚合酶链反应 (qPCR) 显示基因，包括绒毛膜蛋白 S38 样和Rop被Wolbachia下调。的RNA干扰（RNAi）样S38绒毛膜蛋白和罗普在Wolbachia-未感染T.截产卵下降，这与一致的沃尔巴克氏体诱导的产卵减少。有趣的是，在Wolbachia感染的T. truncatus 中抑制Rop导致鸡蛋中Wolbachia滴度增加；然而，在绒毛膜蛋白 S38 样的RNAi 之后，这并没有发生。这是第一项表明绒毛膜蛋白 S38 样和Rop促进Wolbachia-T. truncatus生育力的介导变化。此外，RNA干扰罗普转向的车身颜色沃尔巴克氏体-uninfected T.截黑色，这表明的作用罗普不限于生殖调控T.截。
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