Xylooligosaccharide (XOS) liquor, enzymatically obtained from almond‐shell hemicellulose and consisting primarily of xylobiose and xylotriose, was refined and concentrated using a combination of membranes and ion‐exchange resin. The concentrated liquor was used for an in vitro fermentation study utilizing different strains of Lactobacillus and Bifidobacterium. Upon ultrafiltration with a 10 kDa membrane, high molecular‐weight components, including enzymes and color‐imparting impurities, could be removed from the liquor. Upon nanofiltration with a 150 Da membrane, at a volume:concentration ratio of 8.9, a retentate containing 79% xylobiose and 41.3% xylotriose was obtained. At the end of filtration, 69.6 ± 3.8% of the initial XOS was recovered, with concentrated xylobiose and xylotriose in the retentate. The resin treatment further improved the purity of the XOS concentrate via decoloration and deacidification. Among the evaluated strains of Lactobacillus and Bifidobacterium, L. acidophilus, B. adolescentis, and B. brevis could ferment XOS to a varying extent, as indicated by their difference in growth, to produce acetate as a predominant short‐chain fatty acid. For Lactobacillus, a 10–12‐fold increase in the bacterial population was observed at the end of 48 h. On the other hand, Bifidobacterium grew slowly to show about a 1.2 to fourfold increase in the bacterial population at the end of 72 h. A bacterial preference for fermenting xylobiose rather than xylotriose or xylotetrose was also observed, justifying the production of XOS with a low degree of polymerization. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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使用膜的低聚合度低聚木寡糖及其对细菌发酵的影响

低聚木糖（XOS）酒，是从杏仁壳半纤维素中酶促获得的，主要由木糖和木三糖组成，使用膜和离子交换树脂的组合进行精制和浓缩。浓缩液用于利用乳酸杆菌和双歧杆菌的不同菌株进行的体外发酵研究。。用10 kDa膜超滤后，可以从液体中除去高分子量成分，包括酶和赋予颜色的杂质。在以150Da的膜进行纳滤后，以体积：浓度比为8.9，获得了含有79％的木糖和41.3％的木糖的渗余物。过滤结束时，回收了最初XOS的69.6±3.8％，渗余物中含有浓缩的木糖和木三糖。树脂处理通过脱色和脱酸进一步提高了XOS浓缩液的纯度。在乳酸菌和双歧杆菌的评估菌株中，嗜酸乳杆菌，青春芽孢杆菌和短芽孢杆菌B.可以通过XOS的生长差异将XOS发酵到不同的程度，从而产生乙酸盐作为主要的短链脂肪酸。对于乳酸杆菌，在48小时结束时观察到细菌种群增加了10-12倍。另一方面，双歧杆菌生长缓慢，在72 h结束时细菌数量增加了约1.2至4倍。还观察到细菌偏爱发酵木二糖而不是木三糖或木糖，这证明了低聚合度生产XOS是合理的。©2020年化学工业协会和John Wiley＆Sons，Ltd