Transcription Factor Based Small-Molecule Sensing with a Rapid Cell Phone Enabled Fluorescent Bead Assay.,Angewandte Chemie International Edition

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Transcription Factor Based Small-Molecule Sensing with a Rapid Cell Phone Enabled Fluorescent Bead Assay.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-09-18 , DOI: 10.1002/anie.202007575
Margaret Chern ₁ , Padric M Garden ₂ , R C Baer ₃ , James E Galagan _{2,

3,

4} , Allison M Dennis _{1,

2}

Affiliation

Recently, allosteric transcription factors (TFs) were identified as a novel class of biorecognition elements for in vitro sensing, whereby an indicator of the differential binding affinity between a TF and its cognate DNA exhibits dose‐dependent responsivity to an analyte. Described is a modular bead‐based biosensor design that can be applied to such TF‐DNA‐analyte systems. DNA‐functionalized beads enable efficient mixing and spatial separation, while TF‐labeled semiconductor quantum dots serve as bright fluorescent indicators of the TF‐DNA bound (on bead) and unbound states. The prototype sensor for derivatives of the antibiotic tetracycline exhibits nanomolar sensitivity with visual detection of bead fluorescence. Facile changes to the sensor enable sensor response tuning without necessitating changes to the biomolecular affinities. Assay components self‐assemble, and readout by eye or digital camera is possible within 5 minutes of analyte addition, making sensor use facile, rapid, and instrument‐free.

中文翻译：

基于转录因子的小分子传感与快速手机启用的荧光珠测定。

最近，变构转录因子 (TFs) 被鉴定为一类用于体外传感的新型生物识别元件，其中 TF 与其同源 DNA 之间不同结合亲和力的指标对分析物表现出剂量依赖性响应。描述了一种基于微珠的模块化生物传感器设计，可应用于此类 TF-DNA 分析物系统。DNA 功能化珠子可实现有效混合和空间分离，而 TF 标记的半导体量子点可作为 TF-DNA 结合（珠子上）和未结合状态的明亮荧光指示剂。抗生素四环素衍生物的原型传感器表现出纳摩尔灵敏度，可对珠子荧光进行视觉检测。对传感器的轻松更改使传感器响应调整成为可能，而无需更改生物分子亲和力。

更新日期：2020-11-16

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