This study is aimed to investigate the antifungal activity of endophytic fungi isolated from Jerusalem artichoke (Helianthus tuberosus) and Ginger (Zingiber officinale) and Stemona root (Stemona tuberosa) against causative agent of southern stem rot Sclerotium rolfsii. The dual assay results showed that out of 110 fungal endophytes, Diaporthe phaseolorum BUP3/1 and Macrophomina phaseolina BUP2/3, isolated from Jerusalem artichoke and Daldinia eschscholtzii 2NTYL11 and Trichoderma erinaceum ST-KKU2, isolated from Stemona root and ginger could effectively inhibited the mycelial growth of S. rolfsii at 76.00, 41.20, 66.67 and 63.63%, respectively. The β-1,3 glucanase and chitinase activities of all endophytic fungi was determined which reached the maximum after 2 and 7 days of incubation, respectively. In addition, Scanning Electron Microscope (SEM) observation showed that T. erinaceum ST-KKU2 has predatory mechanism aginst S. rolfsii via coiling and invasion. Interestingly, our endophytic fungi T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 were the first species in their genera to produce polyketides group as 6-n-pentyl-2H-pyran-2-one (6PAP) and 2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one (DHMB), respectively, to control S. rolfsii. Moreover, the antagonistic mechanisms of all endophytes were determined in vivo under greenhouse condition. The endophytic fungi, T. erinaceum ST-KKU2 and D. eschscholtzii 2NTYL11 also showed high level of reduction of southern stem rot disease by 58.14% under pot experiment. Such effects were also higher than the effects of other endophytic fungi isolated from Jerusalem artichoke when tested under greenhouse condition. Our experiments showed the fungal endophytes isolated have potential for further development as a biocontrol agent in the future.

本研究旨在探讨菊芋（内生真菌的抗真菌活性菊芋）和姜（生姜）和百部（百部）针对南方茎腐病的病原体白绢病。双重测定结果表明，从菊芋和Daldinia eschscholtzii 2NTYL11和毛木霉ST-KKU2分离的110种真菌内生菌中，从菊苣根和生姜中分离出的菜豆Diaporthe phaseolorum BUP3 / 1和Macrophomina phaseolina BUP2 / 3可以有效抑制菌丝体。罗非鱼的生长分别为76.00、41.20、66.67和63.63％。确定所有内生真菌的β-1,3葡聚糖酶和几丁质酶活性，分别在孵育2天和7天后达到最大值。此外，扫描电子显微镜（SEM）观察表明，T. erinaceum ST-KKU2具有掠夺机构aginst白绢病菌经由卷取和侵袭。有趣的是，我们的内生真菌T. erinaceum ST-KKU2和D. eschscholtzii 2NTYL11在他们属，以产生聚酮化合物组的第一个物种6- ñ -戊基-2H-吡喃-2-酮（6PAP）和2,3-二氢-5-羟基-2-甲基-4H-1-苯并吡喃-4-酮（DHMB），分别控制白绢病菌。此外，在温室条件下在体内确定了所有内生菌的拮抗机制。内生真菌，T. erinaceum ST-KKU2和D. eschscholtzii 2NTYL11也显示减少南方茎腐病的高级别由58.14％下盆栽试验。在温室条件下进行测试时，这种效果也高于从菊芋中分离出来的其他内生真菌的效果。我们的实验表明，分离出的真菌内生菌有可能在将来作为生物防治剂进一步发展。