In this study, we present the microfabrication and characterization of a transparent microelectrode array (MEA) system based on PEDOT:PSS for electrophysiology. The influence of the PEDOT:PSS electrode dimensions on the impedance was investigated and the stability over time under physiological environment was demonstrated. A very good transparency value was obtained by our system displaying one of the best impedance and transmittance values when compared to other transparent MEA. After biocompatibility validation, we successfully recorded spontaneous neuronal activity of primary cortical neurons cultured over 4 weeks on the transparent PEDOT:PSS electrodes. This work shows that microelectrodes composed of PEDOT:PSS are very promising as a new tool for both electrophysiology and fluorescence microscopy studies on neuronal cell cultures.

在这项研究中，我们介绍基于电生理的PEDOT：PSS的透明微电极阵列（MEA）系统的微细加工和表征。研究了PEDOT：PSS电极尺寸对阻抗的影响，并证明了在生理环境下随时间的稳定性。通过与其他透明MEA相比，我们的系统显示出最佳的阻抗和透射率值之一，因此获得了非常好的透明度值。经过生物相容性验证后，我们成功记录了在透明PEDOT：PSS电极上培养4周以上的原代皮层神经元的自发神经元活性。这项工作表明，由PEDOT：PSS组成的微电极作为用于神经元细胞培养物的电生理学和荧光显微镜研究的新工具非常有前途。