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CRISPR/Cas9 targeted mutagenesis of SlLBD40, a Lateral Organ Boundaries Domain transcription factor, enhances drought tolerance in tomato
Plant Science ( IF 4.2 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.plantsci.2020.110683
Lun Liu , Jialong Zhang , Jiayi Xu , Yafei Li , Luqin Guo , Zhirong Wang , Xichun Zhang , Bing Zhao , Yang-Dong Guo , Na Zhang

The LATERAL ORGAN BOUNDARIES DOMAIN (LBD)-containing genes are plant-specific genes that play important roles in lateral organ development. In this study, we identified LBD40 (Solyc02g085910), which belongs to subfamily II of the LBD family of genes in tomato. LBD40 was highly expressed in roots and fruit. LBD40 expression was significantly induced by PEG and salt. Moreover, SlLBD40 expression was induced by methyl jasmonate treatment, while SlLBD40 expression could not be induced in the jasmonic acid-insensitive1 (jai1) mutant or MYC2-silenced plants, in which jasmonic acid (JA) signaling was disrupted. These findings demonstrate that SlLBD40 expression was dependent on JA signaling and that it might be downstream of SlMYC2, which is the master transcription factor in the JA signal transduction pathway. Overexpressing and CRISPR/Cas9 mediated knockout transgenic tomato plants were generated to explore SlLBD40 function. The drought tolerance test showed that two SlLBD40 knockout lines wilted slightly, while SlLBD40 overexpressing plants suffered severe wilting. The statistical water loss rate and midday leaf water potential also confirmed that knockout of SlLBD40 improved the water-holding ability of tomato under drought conditions. Taken together, our study demonstrates that SlLBD40, involved in JA signaling, was a negative regulator of drought tolerance and that knockout of SlLBD40 enhanced drought tolerance in tomato. This study also provides a novel function of SlLBD40, which belongs to subfamily II of LBD genes.

中文翻译:

CRISPR/Cas9 靶向诱变 SlLBD40(一种横向器官边界域转录因子)增强番茄的耐旱性

包含横向器官边界域 (LBD) 的基因是植物特异性基因,在横向器官发育中起重要作用。在这项研究中,我们鉴定了 LBD40 (Solyc02g085910),它属于番茄 LBD 基因家族的亚家族 II。LBD40 在根和果实中高度表达。PEG和盐显着诱导LBD40表达。此外,茉莉酸甲酯处理诱导了 SlLBD40 表达,而茉莉酸不敏感 1 (jai1) 突变体或 MYC2 沉默植物中不能诱导 SlLBD40 表达,其中茉莉酸 (JA) 信号传导被破坏。这些发现表明 SlLBD40 表达依赖于 JA 信号传导,并且它可能位于 SlMYC2 的下游,SlMYC2 是 JA 信号转导途径中的主要转录因子。产生过表达和 CRISPR/Cas9 介导的敲除转基因番茄植物以探索 SlLBD40 功能。耐旱性试验表明,两个 SlLBD40 基因敲除株系轻微枯萎,而 SlLBD40 过表达的植株枯萎严重。统计失水率和正午叶水势也证实敲除 SlLBD40 提高了番茄在干旱条件下的持水能力。总之,我们的研究表明,参与 JA 信号传导的 SlLBD40 是耐旱性的负调节因子,并且敲除 SlLBD40 增强了番茄的耐旱性。该研究还提供了属于 LBD 基因亚家族 II 的 SlLBD40 的新功能。耐旱性试验表明,两个 SlLBD40 基因敲除株系轻微枯萎,而 SlLBD40 过表达的植株枯萎严重。统计失水率和正午叶水势也证实敲除 SlLBD40 提高了番茄在干旱条件下的持水能力。总之,我们的研究表明,参与 JA 信号传导的 SlLBD40 是耐旱性的负调节因子,并且敲除 SlLBD40 增强了番茄的耐旱性。该研究还提供了属于 LBD 基因亚家族 II 的 SlLBD40 的新功能。耐旱性试验表明,两个 SlLBD40 基因敲除株系轻微枯萎,而 SlLBD40 过表达的植株枯萎严重。统计失水率和正午叶水势也证实敲除 SlLBD40 提高了番茄在干旱条件下的持水能力。总之,我们的研究表明,参与 JA 信号传导的 SlLBD40 是耐旱性的负调节因子,并且敲除 SlLBD40 增强了番茄的耐旱性。该研究还提供了属于 LBD 基因亚家族 II 的 SlLBD40 的新功能。统计失水率和正午叶水势也证实敲除 SlLBD40 提高了番茄在干旱条件下的持水能力。总之,我们的研究表明,参与 JA 信号传导的 SlLBD40 是耐旱性的负调节因子,并且敲除 SlLBD40 增强了番茄的耐旱性。该研究还提供了属于 LBD 基因亚家族 II 的 SlLBD40 的新功能。统计失水率和正午叶水势也证实敲除 SlLBD40 提高了番茄在干旱条件下的持水能力。总之,我们的研究表明,参与 JA 信号传导的 SlLBD40 是耐旱性的负调节因子,并且敲除 SlLBD40 增强了番茄的耐旱性。该研究还提供了属于 LBD 基因亚家族 II 的 SlLBD40 的新功能。
更新日期:2020-12-01
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