Abstract In this study, 189 strains of rice blast fungus isolated from rice-growing fields in five counties within the Jiangxi Province, China, were amplified with 16 pairs of virulence gene-specific primers. Fifteen pairs were able to amplify their target bands. The percentage of polymorphic sites was as high as 93.75%, and the frequency of amplification varied greatly. Cluster analysis classified the 189 strains, represented by 108 different haplotypes, into 21 genetic lineages at a genetic similarity level of 0.80. JXH16 was the dominant haplotype. The 21 genetic lineages included 1 dominant, 2 subdominant, 12 secondary, and 6 minor lineages. At the population level, rich genetic diversity was observed among the 189 strains from different geographic regions (H = 0.2890; I = 0.4398). At a genetic distance of 0.02, the five populations were clustered into three groups that were unrelated to geographical distribution. A certain level of genetic differentiation was found to exist within each blast fungus population (HT = 0.2842). Within-population genetic diversity was higher than that among populations (Hs = 0.2210; Dst = 0.0632), with 77.75% of the total genetic variation originates within populations (Gst = 0.2225). Some within-population gene flow were detected (Nm = 1.7473). The results of our study, which revealed the genetic structure and diversity of rice blast fungal strains in five major rice cultivation areas of Jiangxi Province and their relationship to geographical distribution, can serve as a basis for rice blast resistance breeding and the selection of suitable varieties for these regions, including Duchang County, Jinggangshan City, Fengcheng City, Wan ‘an County and Wuyuan County.

中文翻译：

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基于毒力相关基因指纹图谱的稻瘟病菌种群间遗传多样性

摘要 本研究利用16 对毒力基因特异引物对189 株从江西省5 个县的稻田中分离出来的稻瘟病菌进行扩增。15 对能够放大他们的目标条带。多态位点比例高达93.75%，扩增频率差异较大。聚类分析以 0.80 的遗传相似度将 189 个菌株（由 108 个不同的单倍型代表）分为 21 个遗传谱系。JXH16 是显性单倍型。21个遗传谱系包括1个显性、2个次显性、12个次要和6个次要谱系。在种群水平上，来自不同地理区域的 189 个菌株之间观察到丰富的遗传多样性（H = 0.2890；I = 0.4398）。在遗传距离为 0.02 时，这五个人群被分为三组，与地理分布无关。发现每个稻瘟病菌种群中都存在一定程度的遗传分化（HT = 0.2842）。种群内遗传多样性高于种群间遗传多样性（Hs = 0.2210；Dst = 0.0632），77.75%的遗传变异来自种群内（Gst = 0.2225）。检测到一些种群内基因流（Nm = 1.7473）。我们的研究结果揭示了江西省五个主要水稻种植区的稻瘟病真菌菌株的遗传结构和多样性及其与地理分布的关系，可为稻瘟病抗性育种和适宜品种选择提供依据。这些地区，包括井冈山市都昌县，