Environmental pollution with heavy metals becomes an issue of serious concern worldwide. Cadmium is considered one which adversely affects living organisms. Recently, the usage of endophytic bacteria to enhance the plant growth and phytoremediation of heavy metals contaminated sites is gaining great attention. The current study focused on utilizing the spores of Bacillus megaterium BM18-2 as biofertilizer for enhancing the growth of Cd hyperaccumulator Hybrid Pennisetum and Cd tolerance of the plant. Therefore, the production of the highest proportion of BM18-2 spores in short incubation time was investigated using different culture media. The results revealed that the maximum proportion of BM18-2 spores (90%) was obtained following incubation for 48 h in Tryptone- yeast extract media (TY). Furthermore, several growth parameters of H. Pennisetum were shown to be significantly improved by inclusion of BM18-2 spores into Cd contaminated soil in contrast to non- inoculated plant. The chlorophyll concentration of the leaves rose by 5%, 13%, and 22.89% with increasing Cd concentration of soil (20, 40 and 60 mg/Kg, respectively). The percentage of total nitrogen content of the root, stem and leaf was increased due to the bacterial spores inoculation and the highest percentage was recorded in the leaf in all treatments. Moreover, Cd phytoremediation capacity of H. Pennisetum greatly enhanced with the application of BM18-2 spores into the soil. An obvious correlation was also observed between Cd accumulation and bacterial colonization where the Cd accumulation enhanced by 21.9%, 16.5%, and 94.6% and the maximum count of BM18-2 (27 × 10⁵, 194 × 10⁴,and 145 × 10⁴ CFU/g) were recorded in the root system in 20, 40, and 60 mg/Kg Cd spiked soil, respectively. Consequently, the spores of BM18-2 was proven to succeed as biofertilizer to improve growth of H. pennisetum during Cd stress which subsequently improved the phytoremediation of Cd contaminated soil.

重金属对环境的污染已成为世界范围内严重关注的问题。镉被认为会对生物产生不利影响。近来，使用内生细菌来促进植物生长和被重金属污染部位的植物修复受到极大关注。目前的研究集中在利用巨大芽孢杆菌BM18-2的孢子作为生物肥料来促进镉超积累杂种狼尾草的生长。和镉的耐受性。因此，使用不同的培养基研究了在短孵育时间内产生最高比例的BM18-2孢子的情况。结果显示，在胰蛋白yeast-酵母提取物培养基（TY）中孵育48小时后，可获得最大比例的BM18-2孢子（90％）。此外，狼尾草的几个生长参数与未接种植物相比，通过将BM18-2孢子包含在Cd污染的土壤中，可显着改善玉米芽孢病。随着土壤镉含量的增加（分别为20、40和60 mg / Kg），叶片的叶绿素浓度分别增加了5％，13％和22.89％。根部，茎和叶中总氮含量的百分比由于细菌孢子的接种而增加，并且在所有处理中叶中的最高氮含量都记录在案。此外，通过将BM18-2孢子施用到土壤中，狼尾草的Cd植物修复能力大大提高。在Cd积累和细菌定殖之间也发现了明显的相关性，其中Cd积累增加了BM18-2的21.9％，16.5％和94.6％和最大计数（27×10 ⁵分别在20、40和60 mg / Kg Cd加标土壤中的根系中记录到194×10 ⁴和145×10 ⁴ CFU / g）。因此，证明了BM18-2的孢子作为生物肥料成功地改善了Cd胁迫期间狼尾草的生长，从而改善了Cd污染土壤的植物修复。