Baicalein ameliorates ionizing radiation-induced injuries by rebalancing gut microbiota and inhibiting apoptosis.,Life Sciences

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Baicalein ameliorates ionizing radiation-induced injuries by rebalancing gut microbiota and inhibiting apoptosis.
Life Sciences ( IF 5.2 ) Pub Date : 2020-09-18 , DOI: 10.1016/j.lfs.2020.118463
Meifang Wang ₁ , Yinping Dong ₁ , Jing Wu ₁ , Hongyan Li ₁ , Yuanyang Zhang ₁ , Saijun Fan ₁ , Deguan Li ₁

Affiliation

Aims

Ionizing radiation (IR) induces injuries to the hematopoietic and intestinal systems, which are the leading cause of death. Baicalein, a plant-derived flavonoid, shows anti-oxidative stress, anti-apoptosis, anti-inflammation effects in many diseases. In this study, we evaluated the effects and mechanism of baicalein on IR induced intestinal and hematopoietic injuries.

Main methods

Mice were divided into three groups: Control, IR and IR + Baicalein. All of mice were intraperitoneally administered with 100 mg/kg baicalein or normal saline for 1 h before IR, and then a day post-IR. The changes in intestinal structure, function and molecular expression were observed by pathological experiments and western blot. 16S rRNA gene sequencing was performed to analyze gut microbiota and further predicted metabolic pathways through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Hematopoietic function was evaluated by peripheral blood cells count and by flow cytometry analysis of hematopoietic cells composition.

Key findings

Baicalein improved intestinal structure and the ability of proliferation and regeneration after mice exposed to IR, in which the rebalance of gut microbial composition played an important role. KEGG results showed that p53-related apoptotic pathways played important roles in the composition changes of gut microbiota. Then we observed that baicalein inhibited the activation of p53 and p53 mediated mitochondrial apoptosis and death receptor apoptosis in the intestine. In addition, IR induced injuries to hematopoietic system also could be ameliorated by baicalein.

Significance

These results provide new insights into the mechanism of baicalein and support the potential of baicalein as a radioprotective medicine.

中文翻译：

黄ical素通过重新平衡肠道菌群并抑制细胞凋亡来改善电离辐射引起的伤害。

目的

电离辐射（IR）会导致造血和肠道系统受伤，这是死亡的主要原因。黄ical素是植物来源的类黄酮，在许多疾病中均表现出抗氧化应激，抗凋亡和抗炎作用。在这项研究中，我们评估了黄ical素对IR引起的肠道和造血损伤的作用和机制。

主要方法

将小鼠分为三组：对照组，IR和IR +黄ical苷。在IR之前，然后在IR后一天，对所有小鼠腹膜内给予100mg / kg黄mg素或生理盐水。通过病理实验和蛋白质印迹观察肠道结构，功能和分子表达的变化。进行了16S rRNA基因测序，以分析肠道菌群，并通过《京都基因与基因组百科全书》（KEGG）分析进一步预测了代谢途径。通过外周血细胞计数和通过流式细胞术分析造血细胞组成来评估造血功能。

主要发现

黄ical素改善了小鼠暴露于红外线后的肠结构以及增殖和再生能力，其中肠道微生物组成的重新平衡起着重要作用。KEGG结果表明，p53相关的凋亡途径在肠道菌群组成变化中起重要作用。然后我们观察到黄ical素抑制了肠道中p53和p53介导的线粒体凋亡以及死亡受体凋亡的激活。此外，黄ical素也可以减轻IR对造血系统的伤害。