Although it has been confirmed that extracellular polymeric substances (EPS) play a critical role in the aggregation of granular sludge, the relative contributions and structural characteristics of different polymer components have not been clarified. Through enzymatic hydrolysis and an aggregation experiment two types of granular sludge with a high aggregation ability (aerobic and anammox granular sludge) were studied. The results showed that the aggregation ability of anammox sludge was higher than that of aerobic granular sludge (AGS), and another discovery is that the aggregation ability of sludge after polysaccharide (PS) hydrolysis was obviously inferior to that after protein (PN) hydrolysis. To go insight into the differences of PS structure and properties, rheology and atomic force microscopy (AFM) were conducted and demonstrated that anammox PS had a higher viscosity, mechanical strength and self-assembly ability (dense three-dimensional structures). Furthermore, size-exclusion chromatography (SEC), multiangle laser light scattering (MALLS), refractive index (RI), FTIR and XPS analyses indicated that compared with AGS PS, anammox PS had a higher molecular weight, branched structure, proportion of hydrogen bonds, and hydrophobic component. This study is helpful for fully understanding the structure and properties of PS and can provide theoretical support for the high aggregation performance of granular sludge.

尽管已经证实细胞外聚合物（EPS）在颗粒污泥的聚集中起关键作用，但尚未弄清不同聚合物组分的相对作用和结构特征。通过酶水解和聚集实验，研究了两种具有高聚集能力的颗粒污泥（好氧和厌氧颗粒污泥）。结果表明，厌氧污泥的团聚能力高于好氧颗粒污泥（AGS），另一个发现是多糖（PS）水解后的污泥的团聚能力明显低于蛋白质（PN）水解后的团聚能力。要深入了解PS结构和属性的差异，进行了流变学和原子力显微镜（AFM），证明了anammox PS具有更高的粘度，机械强度和自组装能力（致密的三维结构）。此外，尺寸排阻色谱（SEC），多角度激光散射（MALLS），折射率（RI），FTIR和XPS分析表明，与AGS PS相比，anammox PS具有更高的分子量，支链结构，氢键比例和疏水成分。这项研究有助于全面了解PS的结构和性能，并为颗粒污泥的高聚集性能提供理论支持。多角度激光散射（MALLS），折射率（RI），FTIR和XPS分析表明，与AGS PS相比，anammox PS具有更高的分子量，支链结构，氢键比例和疏水性成分。这项研究有助于全面了解PS的结构和性能，并为颗粒污泥的高聚集性能提供理论支持。多角度激光散射（MALLS），折射率（RI），FTIR和XPS分析表明，与AGS PS相比，anammox PS具有更高的分子量，支链结构，氢键比例和疏水性成分。这项研究有助于充分了解PS的结构和性质，并为颗粒污泥的高聚集性能提供理论支持。