Wild relatives of crops are often rich in genetic resources and provide great possibilities for crop improvement. Ipomoea pes-caprae is one of the wild relatives of sweet potato and has high salt tolerance. Transcriptomes in the treatment and control groups at various times were sequenced to identify salt tolerance genes and salt response pathways. A total of 40,525 genes were obtained, of which 2478 and 3334 were differentially expressed in the roots and leaves of I. pes-caprae under salt stress, respectively. Identification of candidate genes revealed that the mitogen-activated protein kinase (MAPK) signaling pathway of plants and plant hormone signal transduction participates in the salt signal of I. pes-caprae under salt stress. Homology to ABI2 (HAB2) and Clade A protein phosphatases type 2C (HAI1), which encode two protein phosphatases 2C (PP2C) in the abscisic acid (ABA) signal pathway, were continuously up-regulated upon salt stress, indicating their key role in the salt signal transduction pathway of I. pes-caprae. The expression of EIN3-binding F-box protein 1 (EBF1) in the ethylene signaling pathway was also up-regulated, revealing that the salt tolerance of I. pes-caprae was related to the scavenging of reactive oxygen species (ROS). This study provides insights into the mechanism of salt-tolerant plants and the mining of salt-tolerant genes in sweet potato for the innovation of germplasm resources.

中文翻译：

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RNA-seq揭示了甘薯的野生近缘Ipomoea pes-caprae的耐盐性

作物的野生近缘种往往具有丰富的遗传资源，为作物改良提供了巨大的可能性。Ipomoea pes-caprae 是甘薯的野生近缘种之一，具有较高的耐盐性。对不同时间处理组和对照组的转录组进行测序，以确定耐盐基因和盐反应途径。共获得40,525个基因，其中2478个和3334个分别在盐胁迫下I. pes-caprae的根和叶中差异表达。候选基因的鉴定表明，植物的丝裂原活化蛋白激酶（MAPK）信号通路和植物激素信号转导参与了盐胁迫下I. pes-caprae的盐信号。与 ABI2 (HAB2) 和进化枝 A 蛋白磷酸酶 2C 型 (HAI1) 同源，在脱落酸 (ABA) 信号通路中编码两种蛋白磷酸酶 2C (PP2C) 的 PP2C 在盐胁迫下持续上调，表明它们在 I. pes-caprae 的盐信号转导通路中起关键作用。EIN3-binding F-box protein 1 (EBF1) 在乙烯信号通路中的表达也上调，表明 I. pes-caprae 的耐盐性与活性氧 (ROS) 的清除有关。本研究为了解甘薯耐盐植物的形成机制和甘薯耐盐基因的挖掘，为种质资源的创新提供了依据。EIN3-binding F-box protein 1 (EBF1) 在乙烯信号通路中的表达也上调，表明 I. pes-caprae 的耐盐性与活性氧 (ROS) 的清除有关。本研究为了解甘薯耐盐植物的形成机制和甘薯耐盐基因的挖掘，为种质资源的创新提供了依据。EIN3-binding F-box protein 1 (EBF1) 在乙烯信号通路中的表达也上调，表明 I. pes-caprae 的耐盐性与活性氧 (ROS) 的清除有关。本研究为了解甘薯耐盐植物的形成机制和甘薯耐盐基因的挖掘，为种质资源的创新提供了依据。