Aerobic degradation of 2, 2', 4, 4'-tetrabrominated diphenyl ether (BDE-47) by Pseudomonas aeruginosa YH (P. aeruginosa YH) were investigated in this study. BDE-47 degradation was mainly through the biological action of intracellular enzymes, and the metabolites included debrominated metabolites (BDE-28 and BDE-7), hydroxylated metabolites (6-OH-BDE-47, 5-OH-BDE-47, 2'-OH-BDE-28 and 4'-OH-BDE-17), and brominated phenols (2,4-DBP and 4-BP). P. aeruginosa YH also exhibited exceptional ability to degrade intermediates, and the degradation rates of 50 μg/L BDE-28, BDE-7, and 2,4-DBP were 68.4%, 82.3% and 92.7% on the 5th day, separately. Transcriptome sequencing revealed that 991 genes were up-regulated, and 923 genes were down-regulated in P. aeruginosa YH after exposure to 0.5 mg/L BDE-47 (FDR≤0.001, |log₂Ratio|≥1). The differentially expressed genes were related to transport, metabolism and stress response. Harf inhibitory concentration (IC₅₀) of BDE-47 decreased from 167.5 mg/L to 68.4 mg/L when multidrug efflux pump was inactivated by 20 mg/L andrographolide, indicating that it helped the bacterial tolerance against BDE-47. Moreover, efflux pump inhibition would accelerate the adsorption of BDE-47. The adsorption rate obtained equilibrium at approximately 70% in 2 days, while 5 days in the control group. Degradation efficiency of 2 mg/L BDE-47 decreased from 26.8% to 13.9% when multidrug efflux was suppressed.

铜绿假单胞菌YH（铜绿假单胞菌YH）对2、2'，4、4'-四溴代二苯醚（BDE-47）的需氧降解进行了研究。BDE-47的降解主要是通过细胞内酶的生物作用，代谢物包括脱溴代谢物（BDE-28和BDE-7），羟基化代谢物（6-OH-BDE-47、5-OH-BDE-47、2 '-OH-BDE-28和4'-OH-BDE-17）以及溴化苯酚（2,4-DBP和4-BP）。铜绿假单胞菌YH还显示出优异的降解中间体的能力，第5天50μg/ L BDE-28，BDE-7和2,4-DBP的降解率分别为68.4％，82.3％和92.7％。 。转录组测序显示991个基因被上调，而923个基因被下调。暴露于0.5 mg / L BDE-47（FDR≤0.001，| log ₂比率|≥1）后，铜绿假单胞菌YH 。差异表达的基因与转运，代谢和应激反应有关。当多药外排泵被20 mg / L穿心莲内酯灭活时，BDE-47的抑菌浓度（IC ₅₀）从167.5 mg / L降低至68.4 mg / L，表明它有助于细菌抵抗BDE-47。此外，外排泵抑制将加速BDE-47的吸附。吸附率在2天达到约70％的平衡，而在对照组中则为5天。抑制多药外排时，2 mg / L BDE-47的降解效率从26.8％降至13.9％。