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Transcriptome profiling of Pseudomonas aeruginosa YH reveals mechanisms of 2, 2′, 4, 4′-tetrabrominated diphenyl ether tolerance and biotransformation
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-09-18 , DOI: 10.1016/j.jhazmat.2020.124038
Shaoyu Tang , Hua Yin , Xiaolong Yu , Shuona Chen , Guining Lu , Zhi Dang

Aerobic degradation of 2, 2', 4, 4'-tetrabrominated diphenyl ether (BDE-47) by Pseudomonas aeruginosa YH (P. aeruginosa YH) were investigated in this study. BDE-47 degradation was mainly through the biological action of intracellular enzymes, and the metabolites included debrominated metabolites (BDE-28 and BDE-7), hydroxylated metabolites (6-OH-BDE-47, 5-OH-BDE-47, 2'-OH-BDE-28 and 4'-OH-BDE-17), and brominated phenols (2,4-DBP and 4-BP). P. aeruginosa YH also exhibited exceptional ability to degrade intermediates, and the degradation rates of 50 μg/L BDE-28, BDE-7, and 2,4-DBP were 68.4%, 82.3% and 92.7% on the 5th day, separately. Transcriptome sequencing revealed that 991 genes were up-regulated, and 923 genes were down-regulated in P. aeruginosa YH after exposure to 0.5 mg/L BDE-47 (FDR≤0.001, |log2Ratio|≥1). The differentially expressed genes were related to transport, metabolism and stress response. Harf inhibitory concentration (IC50) of BDE-47 decreased from 167.5 mg/L to 68.4 mg/L when multidrug efflux pump was inactivated by 20 mg/L andrographolide, indicating that it helped the bacterial tolerance against BDE-47. Moreover, efflux pump inhibition would accelerate the adsorption of BDE-47. The adsorption rate obtained equilibrium at approximately 70% in 2 days, while 5 days in the control group. Degradation efficiency of 2 mg/L BDE-47 decreased from 26.8% to 13.9% when multidrug efflux was suppressed.



中文翻译:

铜绿假单胞菌YH的转录组分析揭示了2、2',4、4'-四溴代二苯醚耐受性的机制和生物转化

铜绿假单胞菌YH(铜绿假单胞菌YH)对2、2',4、4'-四溴代二苯醚(BDE-47)的需氧降解进行了研究。BDE-47的降解主要是通过细胞内酶的生物作用,代谢物包括脱溴代谢物(BDE-28和BDE-7),羟基化代谢物(6-OH-BDE-47、5-OH-BDE-47、2 '-OH-BDE-28和4'-OH-BDE-17)以及溴化苯酚(2,4-DBP和4-BP)。铜绿假单胞菌YH还显示出优异的降解中间体的能力,第5天50μg/ L BDE-28,BDE-7和2,4-DBP的降解率分别为68.4%,82.3%和92.7%。 。转录组测序显示991个基因被上调,而923个基因被下调。暴露于0.5 mg / L BDE-47(FDR≤0.001,| log 2比率|≥1)后,铜绿假单胞菌YH 。差异表达的基因与转运,代谢和应激反应有关。当多药外排泵被20 mg / L穿心莲内酯灭活时,BDE-47的抑菌浓度(IC 50)从167.5 mg / L降低至68.4 mg / L,表明它有助于细菌抵抗BDE-47。此外,外排泵抑制将加速BDE-47的吸附。吸附率在2天达到约70%的平衡,而在对照组中则为5天。抑制多药外排时,2 mg / L BDE-47的降解效率从26.8%降至13.9%。

更新日期:2020-09-20
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