4-Hydroxyphenylpyruvatedioxygenase (HPPD) is a Fe(II)/Co(II)-dependent enzyme which has become one of the most effective herbicide targets. HPPD inhibitors have been developed as efficient herbicides for resistant weed control. Developing a method for efficient and rapid HPPD inhibitors detection is still challenging. N-n-butyl-4-methylhydrazinecarbothioamide-1,8-naphthalimide (NMN) was synthesized and used to detect Co²⁺ efficiently with the limit of detection (LOD) of 7.82 nM with a turn-on fluorescence. Herein a novel fluorescent complex, NMN-Co²⁺ was employed to determine HPPD inhibitors which performed a turn-off effect in the sensing process based on the competitive coordination between the probe and HPPD with Co²⁺. The LODs for three commercial triketone HPPD inhibitors (mesotrione, tembotrione and NTBC) were 6.60 nM, 7.37 nM and 10.22 nM with good sensitivity and selectivity. Furthermore, the present probe has potentials to quantitatively detect mesotrione and tembotrione in real samples.

4-羟基苯基丙酮酸双加氧酶（HPPD）是一种依赖Fe（II）/ Co（II）的酶，已成为最有效的除草剂目标之一。HPPD抑制剂已被开发为控制杂草的有效除草剂。研发高效，快速的HPPD抑制剂检测方法仍然具有挑战性。合成了N-正丁基-4-甲基肼甲硫代酰胺-1,8-萘二甲酰亚胺（NMN），并以有效的检测限（LOD）为7.82 nM并利用开启荧光有效地检测Co ²⁺。在本文中，一种新型荧光配合物NMN -Co ²⁺用于确定HPPD抑制剂，该探针基于探针与HPPD与Co ²⁺的竞争性配位，从而在传感过程中起到关闭作用。。三种市售三酮HPPD抑制剂（甲基磺草酮，替康三酮和NTBC）的LOD为6.60 nM，7.37 nM和10.22 nM，具有良好的灵敏度和选择性。此外，本发明的探针具有定量检测真实样品中的甲基磺草酮和替康三酮的潜力。