Abstract Fluoxetine is a selective serotonin re-uptake inhibitor (SSRI) drug, widely used to treat depression, nervous anorexia, autism and bulimia nervosa. The direct electrochemical behaviour of fluoxetine at a glassy carbon electrode (GCE) was studied in simple aqueous solution using voltammetric techniques. Subsequently, a novel molecular imprinted polymer (MIP) modified GCE was fabricated using itaconic acid and N, N-methylene-bis acrylamide as functional monomer and crosslinking agent, respectively, to produce a sensitive, selective fluoxetine sensor. Precipitation polymerization enabled drop coating of reproducible MIP layers on a GCE as a renewable affinity surface for fluoxetine detection. Initial baseline studies undertaken using CV and SWV allowed for rapid optimization of scan conditions and confirming the irreversible nature of the FXT reaction at a bare GCE but DPV finally allowed for the further and necessary high level sensitivity needed for assays. The binding efficiency and selectivity of the MIP affinity sites for fluoxetine were demonstrated using DPV and responses compared with a non-imprinted polymer layer (NIP). Conditions for MIP formation, loading and incubation were optimized to improve the analytical performance. Under optimized conditions, a linear fluoxetine calibration from 4.99 × 10−7 to 3.38 × 10−5 mol L−1 was obtained, with detection and quantification limits of 3.33 × 10−7 mol L−1 (115.01 μg L−1) and 1.11 × 10−6 mol L−1 (383.38 μg L−1), respectively. The sensor was employed to analyse fluoxetine extracted from blood serum.

中文翻译：

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一种新型分子印迹聚合物层电极，用于提高抗抑郁药氟西汀的灵敏度电化学测定

摘要 氟西汀是一种选择性5-羟色胺再摄取抑制剂（SSRI）药物，广泛用于治疗抑郁症、神经性厌食症、自闭症和神经性贪食症。使用伏安法在简单水溶液中研究了氟西汀在玻碳电极 (GCE) 上的直接电化学行为。随后，分别使用衣康酸和 N, N-亚甲基双丙烯酰胺作为功能单体和交联剂制备了一种新型分子印迹聚合物 (MIP) 修饰的 GCE，以生产灵敏、选择性的氟西汀传感器。沉淀聚合能够在 GCE 上滴涂可重复的 MIP 层，作为氟西汀检测的可再生亲和表面。使用 CV 和 SWV 进行的初始基线研究允许快速优化扫描条件并确认 FXT 反应在裸 GCE 的不可逆性质，但 DPV 最终允许测定所需的进一步和必要的高水平灵敏度。与非印迹聚合物层 (NIP) 相比，使用 DPV 和响应证明了 MIP 亲和位点对氟西汀的结合效率和选择性。优化了 MIP 形成、加载和孵育的条件以提高分析性能。在优化条件下，获得了从 4.99 × 10−7 到 3.38 × 10−5 mol L−1 的线性氟西汀校准，检测和定量限为 3.33 × 10−7 mol L−1 (115.01 μg L−1) 和分别为 1.11 × 10−6 mol L−1 (383.38 μg L−1)。