Pyrite, a natural ore was introduced into constructed wetlands (CWs) to intensify the denitrification and phosphorus deposition in cold climate conditions. Here, CWs with matrices of pyrite/volcanic rock ratios (m/m) of 0:1(CW–V), 1:10 (CW–P1), 1:5 (CW–P2), 1:1 (CW–P3) and 2:1 (CW–P4) were set up. Compared with the control group (CW–V), CW-P_1-4 produced a gradual increase of nitrate-nitrogen (NO₃⁻-N) and total nitrogen (TN) removal by 6.4–19.9% and 6.2–17.5%, respectively, under a 24 h-hydraulic retention time (HRT). Accordingly, the pyrite based autotrophic denitrification (PAD) contributed to 6.7–24.7% of the TN removal in CW-P_1-4. For phosphate-phosphorus (PO₄^3--P) and total phosphorus (TP) removal, CW-P3 and CW-P4 shared the highest efficiency, which was approximately 60% higher than that in CW-V. The main deposition form of phosphorus in CW-P_1-4 was iron and aluminium bound phosphorus (Fe/Al–P), which accounted for 43.2–74.2%. By contrast, calcium and magnesium bound phosphorus (Ca/Mg–P) was the major form in CW-V. In addition, sequencing data implied that Simplicispira was the most abundant heterotrophic denitrifiers in CWs in cold climate. Thiobacillus, which is involved in PAD, was highly enriched only in the rhizosphere (6.9%) and matrix (7.3%) of CW with pyrite.

黄铁矿是一种天然矿石，被引入人工湿地（CWs）中，以增强寒冷气候条件下的反硝化作用和磷的沉积。在这里，黄铁矿/火山岩比（m / m）为0：1（CW–V），1：10（CW–P1），1：5（CW–P2），1：1（CW– P3）和2：1（CW-P4）已设置。与对照组（CW–V）相比，CW-P _1-4的硝态氮（NO ₃ ^-- N）和总氮（TN）去除率逐渐增加了6.4–19.9％和6.2–17.5％，分别在24小时的液压停留时间（HRT）下。因此，基于黄铁矿的自养反硝化（PAD）贡献了CW-P _1-4中TN去除的6.7–24.7％。对于磷酸盐-磷（PO ₄ ^3--P）和总磷（TP）去除，CW-P3和CW-P4的效率最高，比CW-V高约60％。CW-P _1-4中磷的主要沉积形式为铁和铝结合的磷（Fe / Al-P），占43.2-74.2％。相反，钙和镁结合的磷（Ca / Mg–P）是CW-V的主要形式。此外，测序数据暗示Simplicispira在人工湿地中最丰富的异养反硝化菌在寒冷的气候。参与PAD的硫杆菌仅在黄铁矿中CW的根际（6.9％）和基质（7.3％）中高度富集。