Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic tree. Here, 437 LRR-containing proteins containing nine types of LRR domains were identified in V. fordii genome. Phylogenetic analysis suggested that nine types of LRR domains could not be divided into separate classes, implying that these LRR domains had a common origin. Totally, 27 LRRs were related to possible resistance to Fusarium wilt after 2, 8, and 13 days post-inoculation. We further found that Vf06G1605 was up-regulate under Fusarium wilt infection after these three time points, Vf10G1602 and Vf02G1413 were up-regulated at 8, and 13 dpi, while Vf07G2320 was down-regulated at these three time points. The WGCNA and promoter elements suggested that WRKY possibly regulate the responses of LRRs to Fusarium wilt infection. This study highlighted the phylogeny and function of LRRs in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. Our results presented here might clearly illustrate physiological mechanisms of resistance to Fusarium wilt infection and the target of marker-assisted breeding in V. fordii.

据报道，富含亮氨酸的重复序列基因（LRR）在疾病反应中起着重要作用。但是，我们对LRRs对桐树（Vernicia fordii）枯萎病感染的反应了解甚少，桐树是一种重要的经济树。在这里，在佛氏弧菌基因组中鉴定出包含九种类型的LRR结构域的437个含LRR的蛋白质。系统发育分析表明，不能将9种类型的LRR域分为不同的类别，这意味着这些LRR域具有共同的起源。总共有27个LRR与接种后2天，8天和13天后可能对枯萎病的抗性有关。我们进一步发现Vf06G1605在这三个时间点后，Fusarium在镰刀菌枯萎病感染下被上调，Vf10G1602和Vf02G1413在8和13 dpi时被上调，而Vf07G2320在这三个时间点被下调。WGCNA和启动子元件表明WRKY可能调节LRR对镰刀菌枯萎病感染的反应。这项研究突出了系统发育和功能LRR的在油桐和提供这些基因在系统的分析油桐基因组。我们在这里提出的结果可能清楚地说明了抗镰刀菌的生理机制青枯病和福特辅助标记育种的目标。