The extensive use of synthetic insecticides to control vector-borne diseases has caused increased insect resistance, adverse health effects on humans and the ecosystem. Currently, a new nanotechnology strategy is being considered to develop new green-nano-friendly insecticides. This is the first study for the formulation of Mentha spicata essential oil (EO) nanoemulsion and its application against Culex pipiens, and housefly, Musca domestica. Acute oral toxicity of nano and normal emulsions was studied on rats. The results of gas chromatography–mass spectroscopy (GC–MS) analysis showed that the total identified compounds 95.18 % comprised 28 compounds. These included 1,8-Cineole (19.55 %), menthol (14.4 %), 1-p-menthen-8-yl acetate (6.42 %), 4-terpineol (6.28 %), α-terpineol (5.38 %), α-pinene (5.26 %), β-pinene (4.72 %),p-cymene (3.52 %) and delta 3-carene (3.09 %) as the major components. Nanoemulsion with droplet size 97.8 nm was obtained by ultrasonic emulsification for 10 min at ratio 1:1 (v/v) of mint EO and Tween 80. Emulsification conditions and nanoemulsion droplet size are correlated. Mint nanoemulsion showed a high larvicidal activity against C. pipiens (LC₅₀, 43.57 μg/mL) and M. domestica (LC₅₀, 65.13 μg/mL) compared with normal oil and lambda-cyhalothrin insecticide. Nanoformulation increased efficiency by 71.46 % and 52.0 % against C. pipiens and M. domestica, respectively. Nanoformulation had no toxic effects on the treated rats. Mint nanoemulsion is solvent-free, soluble in water, effective, safe, and eco-friendly. Therefore, it can be used to control C. pipiens, and M. domestica larvae as it is or by developing new capsules with controlled-release as a possible product.

合成杀虫剂广泛用于控制媒介传播的疾病，已导致昆虫抗药性增强，对人类和生态系统产生不利的健康影响。当前，正在考虑采用新的纳米技术策略来开发新型的绿色-纳米友好型杀虫剂。这是的制剂中的第一项研究留兰香精油（EO）纳米乳剂和其对应用淡色库蚊，家蝇和，家蝇。研究了纳米和普通乳液对大鼠的急性口服毒性。气相色谱-质谱（GC-MS）分析的结果表明，鉴定出的总化合物中有28种化合物占95.18％。这些包括1,8-Cineole（19.55％），薄荷醇（14.4％），1-对-薄荷8-乙酸乙烯酯（6.42％），4-萜品醇（6.28％），α-萜品醇（5.38％），α -pine烯（5.26％），β-pine烯（4.72％），对-异丙苯（3.52％）和δ3-胡萝卜素（3.09％）是主要成分。通过以薄荷油EO和吐温80的1：1（v / v）的比例进行10分钟的超声乳化，获得液滴尺寸为97.8 nm的纳米乳液。乳化条件与纳米乳液的液滴尺寸相关。薄荷纳米乳剂显示出对高杀幼虫活性C.蚊（LC ₅₀，43.57微克/毫升）和家蝇（LC ₅₀，65.13微克/毫升）与通常的油和λ-氯氟氰菊酯杀虫剂进行比较。纳米配方分别对pipiens和M. domestica的效率提高了71.46％和52.0％。纳米制剂对治疗的大鼠没有毒性作用。薄荷纳米乳液不含溶剂，可溶于水，有效，安全且环保。因此，它可以直接用于防治淡色梭状芽胞杆菌和家蝇的幼虫，也可以通过开发新的胶囊作为可能的产品进行控释。