Sex determination is an important and intriguing research topic in the field of evolutionary and developmental biology. Quantitative trait locus (QTL) mapping for sex is helpful in clarifying the sex determination system of species. In this study, a second high-resolution genetic linkage map was constructed for the ridgetail white prawn, Exopalaemon carinicauda, which included 9280 markers, covering 99.98% of the complete genome. Based on the linkage map, a highly significant sex-related QTL was first mapped to a single linkage group (LG3, LOD > 55.6). Fifty-two markers in the QTL region were significantly associated with sex (p ≤ 10⁻⁴⁰), of which heterogametic genotypes in females supported the ZW sex determination mechanism. Six markers were verified to be significantly associated with sex in the wild population. Some sex-related genes were identified, including phospholipase D, protein kinase shaggy, and longitudinals lacking protein. These results inform our understanding of the mechanisms of sex determination in E. carinicauda.

性别决定是进化和发育生物学领域一个重要而有趣的研究课题。性别的数量性状位点（QTL）定位有助于阐明物种的性别决定系统。本研究构建了脊尾白虾 Exopalaemon carinicauda的第二张高分辨率遗传连锁图谱，其中包括 9280 个标记，覆盖了整个基因组的 99.98%。基于连锁图，首先将一个高度显着的性别相关 QTL 定位到单个连锁群（LG3，LOD > 55.6）。QTL 区域的 52 个标记与性别显着相关（p  ≤ 10 ^-40)，其中雌性异配基因型支持 ZW 性别决定机制。六个标记被证实与野生种群的性别显着相关。鉴定了一些与性别相关的基因，包括磷脂酶 D、蛋白激酶毛茸茸的和缺乏蛋白质的纵向基因。这些结果告诉我们对E. carinicauda性别决定机制的理解。