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Study on the Regulation of Earthworm Physiological Function under Cadmium Stress Based on a Compound Mathematical Model.
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-09-18 , DOI: 10.1016/j.etap.2020.103499
Haoran Zhou 1 , Tingxiu Zhang 1 , Jiayun Zhuang 1 , Mingyuan Xu 1 , Xueli Liu 1 , Qinghua Shi 1 , Dongxing Zhou 2
Affiliation  

A cadmium (Cd) stress test was carried out on Eisenia fetida in artificial soil. Six Cd concentration gradient solutions (0, 50, 100, 125, 250 and 500 mg/kg) were prepared. Two treatment groups, short-term stress and long-term stress, were established. The former lasted for 10 days, and the latter lasted for 30 days. The Biolog ECO-microplate culture method was used to determine the utilization of the 31 carbon sources by the microbes in earthworm homogenate. The total protein content (TP), peroxidase activity (POD), catalase activity (CAT), superoxide dismutase activity (SOD), glutathione peroxidase activity (GPX), glutathione-S-transferase activity (GST), malondialdehyde content (MDA) and acetylcholinesterase activity (AChE) in earthworm were determined in order to investigate the regulation of oxidative stress and the functional diversity of microbial communities in earthworms under Cd stress. By combining the entropy weight method (EW) and the technique for order preference by similarity to an ideal solution model (TOPSIS), the physiological functional indices of earthworms were assessed objectively and scientifically, and the physiological changes under the different stress periods were evaluated. The results showed that a Cd-tolerant dominant population appeared in the microbial community under Cd stress. In the short-term test, oxidative stress were more effective in coping with Cd stress than the microbial community, and oxidative stress regulated the microbial community functional diversity. Under long-term Cd stress, the regulatory effect was weak or non-existent. In this study, a new evaluation model was established to explore the regulation process of earthworm on its oxidation stress and the functional diversity of microbial communities under Cd stress, and provide a theoretical basis for revealing the detoxification mechanism of earthworms.



中文翻译:

基于复合数学模型的镉胁迫下worm生理功能调控的研究。

Fetida上进行了镉(Cd)压力测试在人造土壤中。制备了六种Cd浓度梯度溶液(0、50、100、125、250和500 mg / kg)。建立了短期压力和长期压力两个治疗组。前者持续10天,后者持续30天。Biolog ECO-微孔板培养法用于确定worm匀浆中微生物对31种碳源的利用。总蛋白含量(TP),过氧化物酶活性(POD),过氧化氢酶活性(CAT),超氧化物歧化酶活性(SOD),谷胱甘肽过氧化物酶活性(GPX),谷胱甘肽S-转移酶活性(GST),丙二醛含量(MDA)和测定determined中的乙酰胆碱酯酶活性(AChE),以研究镉胁迫下the的氧化应激调节和微生物群落功能多样性。通过结合熵权法(EW)和类似于理想解模型的TOPSIS技术(TOPSIS),客观科学地评价了the的生理功能指标,并评估了不同胁迫时期的生理变化。结果表明,在镉胁迫下微生物群落中出现了耐Cd的优势种群。在短期测试中,氧化应激比微生物群落更有效地应对Cd胁迫,并且氧化应激调节了微生物群落的功能多样性。在长期的镉胁迫下,调节作用弱或不存在。在这个研究中,

更新日期:2020-10-04
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