Abstract Background Fructose and single cell protein are important products for the food market. Abundant amounts of low-grade dates worldwide are annually wasted. In this study, highly concentrated fructose syrups and single cell protein were obtained through selective fermentation of date extracts by Saccharomyces cerevisiae. Results The effect of air flow (0.1, 0.5, 0.75, 1, 1.25 and 1.5 vvm) and pH (4.5, 4.8, 5, 5.3 and 5.6) was investigated. Higher air flow led to lower fructose yield. The optimum cell mass production of 10 g/L was achieved at air flow of 1.25 vvm with the fructose yield of 91%. Similar cell mass production was obtained in the range pH of 5.0–5.6, while less cell mass was obtained at pH less than 5. Controlling the pH at 4.5, 5.0 and 5.3 failed to improve the production of cell mass which were 5.6, 5.9 and 5.4 g/L respectively; however, better fructose yield was obtained. Conclusions Extension of the modified Gompertz enabled excellent predictions of the cell mass, fructose production and fructose fraction. The proposed model was also successfully validated against data from literatures. Thus, the model will be useful for wide application of biological processes. How to cite: Putra MD, Abasaeed AE, Al-Zahrani SM. Prospective production of fructose and single cell protein from date palm waste. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.09.007 .

中文翻译：

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从椰枣废料中前瞻性生产果糖和单细胞蛋白

摘要 背景果糖和单细胞蛋白是食品市场的重要产品。全世界每年都有大量的低级椰枣被浪费掉。在本研究中，通过酿酒酵母对枣提取物进行选择性发酵，获得了高浓度果糖糖浆和单细胞蛋白。结果 研究了空气流量（0.1、0.5、0.75、1、1.25 和 1.5 vvm）和 pH 值（4.5、4.8、5、5.3 和 5.6）的影响。较高的空气流量导致较低的果糖产量。在 1.25 vvm 的空气流量下实现了 10 g/L 的最佳细胞批量生产，果糖产率为 91%。在 pH 5.0-5.6 范围内获得了类似的细胞团产量，而在 pH 值小于 5 时获得的细胞团较少。将 pH 控制在 4.5、5.0 和 5.3 未能提高细胞团的产量，分别为 5.6、5.9 和5. 分别为4克/升；然而，获得了更好的果糖产量。结论 改良 Gompertz 的扩展能够很好地预测细胞质量、果糖产量和果糖分数。所提出的模型也成功地针对文献数据进行了验证。因此，该模型将有助于生物过程的广泛应用。如何引用：Putra MD、Abasaeed AE、Al-Zahrani SM。从椰枣废料中前瞻性生产果糖和单细胞蛋白。电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.007。该模型将有助于生物过程的广泛应用。如何引用：Putra MD、Abasaeed AE、Al-Zahrani SM。从椰枣废料中前瞻性生产果糖和单细胞蛋白。电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.007。该模型将有助于生物过程的广泛应用。如何引用：Putra MD、Abasaeed AE、Al-Zahrani SM。从椰枣废料中前瞻性生产果糖和单细胞蛋白。电子 J 生物技术 2020；48。https://doi.org/10.1016/j.ejbt.2020.09.007。