Abstract Strontium-substituted hydroxyapatite (SrHAp) materials are known to actively promote bone formation. However, the optimum level of Sr inclusion needed to elicit a physiologically relevant response from bone cells is unclear and can vary dependent on the fabrication process employed. In this work hydroxyapatite (HAp), SrHAp powders (2, 5 and 10 wt% (i.e., 1, 2 and 5 at%) with respect to [Sr/(Sr + Ca) ∙100]), were synthesized with the purity and Sr-substitution was confined in the range of 1–8 wt% (1–4 at%). All SrHAp samples contained rod-like crystals ( 300 nm in length). TEM-EDX confirmed the presence of Sr and maintenance of the HAp lattice structure for both types of crystals. Qualitative in vitro evaluation using primary human ostesoblast cells (HOBC) cultured in contact with the SrHAp over 28 days showed that the presence of Sr (in particular with the highest Sr content) directly promotes the maturation of osteoblasts into osteocytes as compared to the response observed for HAp. As these materials contain no additives other than Sr, the effects observed here can only be attributed to the physiologically important levels of Sr in the samples.

中文翻译：

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锶取代的羟基磷灰石促进原代人成骨细胞成熟

摘要 锶取代羟基磷灰石 (SrHAp) 材料可积极促进骨形成。然而，引发骨细胞生理相关反应所需的 Sr 含量的最佳水平尚不清楚，并且可能因所采用的制造工艺而异。在这项工作中，羟基磷灰石 (HAp)、SrHAp 粉末（2、5 和 10 重量％（即 1、2 和 5 原子％）相对于 [Sr/(Sr + Ca) ∙100]）被合成，纯度为Sr 取代被限制在 1-8 wt% (1-4 at%) 的范围内。所有 SrHAp 样品都含有棒状晶体（长度为 300 nm）。TEM-EDX 证实了 Sr 的存在和两种类型晶体的 HAp 晶格结构的维持。使用与 SrHAp 接触培养超过 28 天的原代人成骨细胞 (HOBC) 进行的定性体外评估表明，与观察到的反应相比，Sr 的存在（特别是 Sr 含量最高的）直接促进成骨细胞成熟为骨细胞对于HAp。由于这些材料除 Sr 外不包含任何添加剂，因此此处观察到的影响只能归因于样品中 Sr 的重要生理水平。