This work presents the synthesis of platinum and silver nanoparticles obtained on a support composed of the S-layer protein (SLP) isolated from Lactobacillus kefiri and polyurethane particles (PU). The support was obtained by adsorption of the protein on the polymer particles in aqueous dispersion. After combining the support with the platinum or silver salts and subsequently reducing with hydrogen, noble metal-based heterogeneous bionanocatalysts were obtained. Platinum or silver nanoparticles with diameters d_n = 4.9 nm (d_s = 4.9 nm; d_v/d_n = 1.05) or d_n = 8.0 nm (d_s = 10.6 nm; d_v/d_n = 1.63), respectively, were observed by TEM on the surface of the protein/polymer supports. Both nanocatalysts showed excellent catalytic activity in the reduction of p-nitrophenol with NaBH₄ at room temperature, with conversions of 100% and 97% for platinum and silver bionanocatalysts, respectively. Noteworthy, both bionanocatalysts maintain their activity until 4 reuse cycles, being the platinum-based system the one showing the best performance. These results demonstrated that the developed bionanocatalysts are promising and emerging alternatives in the field of supported noble metal nanoparticle-based heterogeneous catalysts.

这项工作介绍了在由从牛乳杆菌分离的S层蛋白（SLP）和聚氨酯颗粒（PU）组成的载体上获得的铂和银纳米颗粒的合成。通过将蛋白质吸附在水分散体中的聚合物颗粒上来获得载体。在将载体与铂盐或银盐混合并随后用氢还原之后，获得了基于贵金属的非均相生物仿生催化剂。直径d _n  = 4.9 nm（d _s  = 4.9 nm; d _v / d _n  = 1.05）或d _n  = 8.0 nm（d _s  = 10.6 nm; d _v / d _n的铂或银纳米颗粒 透射电镜分别在蛋白质/聚合物载体表面上观察到= 1.63）。两种纳米催化剂在室温下用NaBH ₄还原对硝基苯酚均表现出出色的催化活性，铂和银离子纳米催化剂的转化率分别为100％和97％。值得注意的是，两种生物纳米催化剂均保持其活性直至4个重复使用周期，这是铂基体系中表现出最佳性能的体系。这些结果表明，在负载型贵金属纳米颗粒基非均相催化剂领域，已开发的生物纳米催化剂是有前途的和新兴的替代品。